THE LDL RECEPTOR GENE - A MOSAIC OF EXONS SHARED WITH DIFFERENT PROTEINS
THE LDL RECEPTOR GENE - A MOSAIC OF EXONS SHARED WITH DIFFERENT PROTEINS
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DOI：
10.1126/science.2988123
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
RUSSELL, DW
中科院分区：
文献类型：
--
作者：
SUDHOF, TC;GOLDSTEIN, JL;RUSSELL, DW
多功能的性质，包被坑受体预测，这些蛋白质将包含多个域。为了建立这些结构域的遗传基础，我们已经确定了低密度脂蛋白（LDL）受体基因的外显子组织。该基因长度超过45个内切酶，包含18个外显子，其中大部分与先前在蛋白质水平上定义的功能结构域相关。18个外显子中的13个编码与其他蛋白质中的序列同源的蛋白质序列：这些外显子中的5个编码与补体的C9组分中的序列相似的序列; 3个外显子编码与表皮生长因子（EGF）前体和凝血系统的3种蛋白质中的重复序列相似的序列（因子IX，因子X和蛋白C）;和其他五个外显子编码仅与EGF前体共享的非重复序列。LDL受体似乎是由不同蛋白质共享的外显子组成的嵌合蛋白，因此它属于几个超基因家族。
The multifunctional nature of coated pit receptors predicts that these proteins will contain multiple domains. To establish the genetic basis for these domains, we have determined the exon organization of the gene for the low-density lipoprotein (LDL) receptor. This gene is more than 45 kilobases in length and contains 18 exons, most of which correlate with functional domains previously defined at the protein level. Thirteen of the 18 exons encode protein sequences that are homologous to sequences in other proteins: five of these exons encode a sequence similar to one in the C9 component of complement; three exons encode a sequence similar to a repeat sequence in the precursor for epidermal growth factor (EGF) and in three proteins of the blood clotting system (factor IX, factor X, and protein C); and five other exons encode nonrepeated sequences that are shared only with the EGF precursor. The LDL receptor appears to be a mosaic protein built up of exons shared with different proteins, and it therefore belongs to several supergene families.