Dibenzothiophene Sulfone Derivatives as Plasma Membrane Dyes
Dibenzothiophene Sulfone Derivatives as Plasma Membrane Dyes
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作为质膜染料的二苯并噻吩砜衍生物
DOI：
10.1111/php.13175
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发表时间：
2019
影响因子：
3.3
通讯作者：
McCulla, Ryan D.
中科院分区：
文献类型：
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作者：
Petroff, II, John T.;Grady, Scott;Arnatt, Christopher K.;McCulla, Ryan D.
二苯并噻吩5，5-二氧化物（DBTOO）衍生物最近已被证明可用作荧光细胞染料。为了扩展基于DBTOO的染料的功能以包括细胞膜的可视化，合成了两种亲脂性DBTOO，并通过荧光显微镜检查了它们掺入HeLa细胞质膜的能力。测定了两种新的DBTOO衍生物的荧光物理性质，它们都具有良好的荧光量子产率和可见的蓝光发射。由于激发和发射波长一致，标准4′，6-二氨基-2-苯基吲哚（DAPI）滤光片组与这些染料配合良好。共染色后，证实两种DBTOO染料均位于质膜中。使用Pearson相关系数量化重叠的质量，这表明DBTOO染料和标准质膜染料之间的强重叠。新染料对HeLa细胞的毒性也相对较低，IC 50在10至100 µm之间。因此，这项工作报告了一个新的用途DBTOO衍生物作为荧光显微镜染色。
Dibenzothiophene 5,5‐dioxide (DBTOO) derivatives have recently been shown to processes utility as fluorescent cell dyes. In an effort to extend the functionality of DBTOO‐based dyes to include the visualization of cellular membranes, two lipophilic DBTOO were synthesized and their ability to incorporate into the plasma membrane of HeLa cells was examined by fluorescent microscopy. The photophysical properties of the two new DBTOO derivatives were determined and both have good fluorescent quantum yields and a visible blue emission. Due to agreeable wavelengths of excitation and emission, a standard 4′,6‐diamindino‐2‐phenylindole (DAPI) filter set worked well with these dyes. After co‐staining, it was confirmed that both DBTOO dyes localized in the plasma membrane. The quality of the overlap was quantified using Pearson correlation coefficient, which indicated a strong overlap between the DBTOO dyes and the standard plasma membrane dye. The novel dyes also displayed relatively low toxicity to the HeLa cells with IC50between 10 and 100 µm. Thus, this work reports a new use of DBTOO derivatives as fluorescent microscopy stains.
芳香亚砜的光化学和光物理学。
DOI：
--
发表时间：
1995
期刊：
影响因子：
--
作者：
Zehong Wan;W. Jenks
通讯作者：
W. Jenks