A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane
A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane
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DOI：
10.1016/j.ajo.2004.11.051
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发表时间：
2005-06-01
影响因子：
4.2
通讯作者：
Ishibashi, T
中科院分区：
文献类型：
--
作者：
Hisatomi, T;Enaida, H;Ishibashi, T
目得：为了研究细胞和细胞外基质对内界膜（ILM）的作用，我们展示了一种新的方法，一个全面的鸟的眼睛分析手术切除的ILM.DESIGN：Laboratory investigation.METHODS：ILM的特发性视网膜前膜固定和传播到载玻片上，使用细针下的生物显微镜。结果：切除的ILM可展开并在载玻片上展开成一个扁平的薄片。免疫组化显示迁移细胞主要为胶质细胞。扫描电镜显示，玻璃体表面的ILM是光滑的，在粗糙的视网膜表面形成对比。ILM表现出丰富的细胞迁移作为一个连续的分层cellularsheet.CONCLUSIONS：这种新的鸟的，切除的ILM的眼睛视图观察，使我们能够进行全面的分析，从时间和空间的角度来看，细胞分布。(c)2005年，Elsevier Inc. All rights reserved.
PURPOSE: To investigate the role of cells and extracellular matrix on the internal limiting membrane (ILM), we demonstrated a new method for a comprehensive bird's, eye analysis of surgically excised ILM.DESIGN: Laboratory investigation.METHODS: The ILM of an idiopathic epiretinal membrane was fixed and spread out onto a glass slide, using fine needles under a biomicroscope. The expanded ILM was analyzed by light microscopy, immunohistochemistry, and scanning electron microscopy.RESULTS: The excised ILM could be unfolded and spread out as a flat sheet onto the glass slide. Immunohistochemistry revealed that most migrating cells were glial cells. Scanning electronmicroscopy revealed that the vitreous surface of the ILM was smooth, in contrast to the rough retinal surface. The ILM showed abundant cellular migration as a continuous stratified cellular sheet.CONCLUSIONS: This new bird's,eye view observation of excised ILM enables us to carry out comprehensive analysis of cellular distribution from a temporal and spatial perspective. (c) 2005 by Elsevier Inc. All rights reserved.