Erythema multiforme lesions are associated with expression of a herpes simplex virus (HSV) gene and qualitative alterations in the HSV-specific T-cell response.
Erythema multiforme lesions are associated with expression of a herpes simplex virus (HSV) gene and qualitative alterations in the HSV-specific T-cell response.
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多形红斑病变与单纯疱疹病毒 (HSV) 基因的表达和 HSV 特异性 T 细胞反应的质量改变有关。
DOI：
10.1046/j.1365-2133.1998.02260.x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Burnett,JW
中科院分区：
文献类型：
--
作者：
Kokuba,H;Imafuku,S;Huang,S;Aurelian,L;Burnett,JW
一种常见形式的多形性红斑，疱疹相关性多形性红斑（HAEM），发生在单纯疱疹病毒（HSV）感染后。在这里，我们报告了HSV基因表达和病毒特异性T细胞反应的定性性质与HAEM病变的发展相关。聚合酶链反应检测HAEM皮损和愈合1-3个月HAEM皮损皮肤的病毒DNA聚合酶基因（Pol）阳性。然而，通过Pol特异性抗体免疫组织化学测定的基因表达仅见于HAEM病变，表明病变发展与Pol基因表达相关。在急性或愈合HAEM病变或HSV病变患者的外周血单核细胞（PBMC）中以及在HSV血清阳性的不相关炎症性疾病患者中观察到相似的HSV特异性T细胞淋巴增殖反应。然而，从HAEM病变中获得的HSV刺激的PBMC的T细胞受体可变（Vβ）链库发生了改变;一些可变链家族（即Vβ16和Vβ19）的患病率降低，而其他家族的患病率升高（即Vβ2和Vβ7）。在Pol抗原阳性的HAEM病变皮肤中发现Vβ2细胞，表明这些细胞归巢于病毒抗原阳性皮肤。
A common form of erythema multiforme, herpes‐associated erythema multiforme (HAEM), occurs following infection with herpes simplex virus (HSV). Here we report that HSV gene expression and the qualitative nature of the virus‐specific T‐cell responses are related to HAEM lesion development. Skin from HAEM lesions and 1–3 months healed HAEM lesional skin were positive for the viral DNA polymerase gene (Pol) by polymerase chain reaction. However, gene expression as determined by immunohistochemistry with Pol‐specific antibody was seen only in HAEM lesions, suggesting that lesion development is associated with Pol gene expression. Similar HSV‐specific T‐cell lymphoproliferative responses were seen in peripheral blood mononuclear cells (PBMCs) from patients with acute or healed HAEM lesions or HSV lesions and from HSV‐seropositive patients with unrelated inflammatory diseases. However, the T‐cell receptor variable (Vβ ) chain repertoire of HSV‐stimulated PBMCs obtained from HAEM lesions was altered; the prevalence of some families of variable chain (namely Vβ16 and Vβ19) was reduced, whereas the prevalence of others was increased (namely Vβ2 and Vβ7). Vβ2 cells were found in HAEM lesional skin positive for Pol antigen, suggesting that these cells home to viral antigen‐positive skin.