Step-by-step design of poly (ε-caprolactone) /chitosan/Melilotus officinalis extract electrospun nanofibers for wound dressing applications
Step-by-step design of poly (ε-caprolactone) /chitosan/Melilotus officinalis extract electrospun nanofibers for wound dressing applications
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DOI：
10.1016/j.ijbiomac.2021.03.046
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发表时间：
2021-03-15
影响因子：
8.2
通讯作者：
Shahrousvand, Mohsen
中科院分区：
文献类型：
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作者：
Shahrousvand, Mohammad;Haddadi-Asl, Vahid;Shahrousvand, Mohsen
在静电纺丝系统中，聚合物的组成和溶剂类型的选择对于获得具有最佳性能的毡是非常重要的。在这项工作中，强调了一种新型溶剂体系的影响，分四个步骤设计了一种电纺伤口敷料。首先，为了研究聚合物-溶剂相互作用和电纺距离的影响，将等量的聚己内酯(PCL)溶解在不同组成的甲酸(FA)/二氯甲烷(DCM)中，并在不同的电纺距离下进行电纺。纤维平均直径最小且表面均匀度较好的最佳工艺条件为80FA/20DCM，纤维间距为15 cm。第二步，通过改变PCL的浓度来实现纤维直径的最小化。最后，在第三步和第四步中，在溶液中加入不同浓度的壳聚糖(CN)和固定剂量的草木香提取物(MO)。含有纤维的提取物的平均直径为275+/-41 nm，符合伤口护理的要求。最后，通过FTIR、DSC、拉伸、水接触角、抗菌试验、细胞活力和药物释放分析对优化后的PCL/CN和PCL/CN/MO样品进行评估，以确定它们的功能和性能。
Composition of polymers and choosing the type of solvents in electrospinning systems is of great importance to achieve a mat with optimal properties. In this work, with emphasizing the influence of a novel solvent system, an electrospun wound dressing was designed in four steps. Firstly, to study the effect of polymer-solvent interactions and electrospinning distance, a constant amount of polycaprolactone (PCL) was dissolved at different compositions of formic acid (FA)/dichloromethane (DCM) and was electrospun at different distances. The composition of 80FA/20DCM and distance of 15 cm were selected as optimal conditions by lowest average diameter of fibers and simultaneously good surface uniformity. In the second step, the concentration of PCL was considered variable to achieve the lowest diameter of fibers. Finally, in the third and fourth steps, different concentrations of chitosan (CN) and constant dosage of Melilotus officinalis (MO) extract were added to the solution. The extract contained fibers had a mean diameter of 275 +/- 41 nm which is in the required condition for wound caring. Eventually, the optimized PCL/CN and PCL/CN/MO specimens were evaluated by FTIR, DSC, Tensile, water contact angle, antibacterial assays, cell viability, and drug release analysis for determining their function and properties.(c) 2021 Elsevier B.V. All rights reserved.