Electroformation of giant liposomes in microfluidic channels
Electroformation of giant liposomes in microfluidic channels
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DOI：
10.1088/0957-0233/17/12/s01
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发表时间：
2006-12-01
影响因子：
2.4
通讯作者：
Takeuchi, S.
中科院分区：
文献类型：
--
作者：
Kuribayashi, K.;Tresset, G.;Takeuchi, S.
我们提出了一种芯片上的方法，利用微流控通道中的电铸法制备各种类型的巨型脂质体。这些通道被夹在涂有氧化铟锡(ITO)电极的玻璃片之间。通过施加交流电压，在通道内形成巨大的脂质体。对所得脂质体的重要性质进行了定量研究。我们发现，电铸法制备的脂质体中，54%的脂质体直径大于10微米，90%的脂质体本身没有包裹额外的脂质体。利用两个微流控通道，我们发现在一个芯片上同时形成了包裹了纳米/微米材料的巨型脂质体。
We present an on-chip method to produce various types of giant liposomes using electroformation in microfluidic channels. These channels were sandwiched between glass slides coated with indium tin oxide (ITO) electrodes. Giant liposomes were formed inside the channels by applying an ac voltage. Important characteristics of the obtained liposomes were investigated quantitatively. We found that 54% of the liposomes produced by electroformation had diameters exceeding 10 mu m and that 90% of the liposomes did not enclose extra liposomes inside themselves. Using two microfluidic channels, we found that giant liposomes with nano/micrometre-sized materials encapsulated were formed simultaneously on a chip.