Toll-like receptor 2-dependent activation of monocytes by Spirulina polysaccharide and its immune enhancing action in mice
Toll-like receptor 2-dependent activation of monocytes by Spirulina polysaccharide and its immune enhancing action in mice
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DOI：
10.1016/j.intimp.2006.08.001
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发表时间：
2006-12-05
影响因子：
5.6
通讯作者：
Pasco, David S.
中科院分区：
文献类型：
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作者：
Balachandran, Premalatha;Pugh, Nirmal D.;Pasco, David S.
我们之前报道过，来自螺旋藻的高分子量多糖组分（Immulina）是NF-κ B的有效激活剂，并诱导THP-1人单核细胞中的IL-1 β和TNF-α mRNA。在本研究中，我们发现免疫素激活的NF-κ B被CD 14和TLR 2抗体抑制，但不被TLR 4抗体抑制。类似地，当用表达支持TLR 2-（CD 14和TLR 2）但不支持TLR 4-（CD 14、TLR 4和MD-2）依赖性活化的蛋白质的载体共转染细胞时，通过Immulina处理增强NF-κ B指导的荧光素酶表达。食用与含有Immulina的提取物混合的化学成分确定的食物的小鼠表现出几个免疫参数的变化。Immulina处理的小鼠中，来自派伊尔斑细胞的伊加和IL-6的离体产生增加2倍，来自脾细胞的干扰素-γ产生增加4倍。这些因子的产生增强在食用该提取物4或5天的小鼠中最为显著。这些研究揭示了Immulina如何激活先天免疫系统的细胞，并表明口服这种多糖可以增强粘膜和全身免疫系统中的成分。(c)2006 Elsevier B. V.保留所有权利。
We reported previously that a high molecular weight polysaccharide fraction (Immulina) from Spirulina was a potent activator of NF-kappa B and induced both IL-1 beta and TNF-alpha mRNAs in THP-1 human monocytes. In the present study, we show that NF-kappa B activation by Immulina is suppressed by antibodies to CD14 and TLR2 but not by antibodies to TLR4. Similarly, NF-kappa B directed luciferase expression was enhanced by Immulina treatment when cells were co-transfected with vectors expressing proteins supporting TLR2- (CD14 and TLR2) but not TLR4-(CD14, TLR4, and MD-2) dependent activation. Mice that consumed a chemically defined chow mixed with an extract containing Immulina exhibited changes in several immune parameters, The ex vivo production of IgA and IL-6 from Peyer's patch cells was enhanced 2-fold and interferon-gamma production from spleen cells was increased 4-fold in Immulina-treated mice. The enhanced production of these factors was most notable with mice that had consumed this extract for 4 or 5 days. These studies shed light on how Immulina activates cells of the innate immune system and suggests that oral consumption of this polysaccharide can enhance components within both the mucosal and systemic immune systems. (c) 2006 Elsevier B.V. All rights reserved.