The 5'region of the rat phosphoenolpyruvate carboxykinase gene confers pH sensitivity to chimeric genes expressed in renal and liver cell lines capable of expressing PEPCK.
The 5'region of the rat phosphoenolpyruvate carboxykinase gene confers pH sensitivity to chimeric genes expressed in renal and liver cell lines capable of expressing PEPCK.
复制标题
大鼠磷酸烯醇丙酮酸羧激酶基因的5区域赋予对能够表达PEPCK的肾和肝细胞系中表达的嵌合基因的pH敏感性。
DOI：
10.1016/0006-291x(89)91685-9
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发表时间：
1989
影响因子：
3.1
通讯作者：
Long,JA
中科院分区：
文献类型：
--
作者：
Pollock,AS;Long,JA
大鼠磷酸烯醇丙酮酸羧激酶基因的5'侧翼区域用于与人生长激素基因形成嵌合基因构建体。这些构建体被转染到几种肾细胞系和一种肝细胞系中，并通过免疫测定法测量生长激素（HGH）的产生。在所有细胞系中均观察到 HGH 产生的环磷酸腺苷和糖皮质激素反应性。在两个细胞系中，大鼠 NRK52E 肾上皮细胞系和大鼠 H4IIE 肝癌细胞系都能够表达 PEPCK，降低细胞外 pH 值，使 HGH 产量增加几倍。比较激素和pH对胸苷激酶启动子-HGH嵌合体转染细胞的影响表明PEPCK 5'侧翼区域的影响是特异性的。因此，PEPCK 基因在体内的部分 pH 响应性可能归因于 5' 侧翼区域的特性。
The 5′ flanking regions of the rat phosphoenolpyruvate carboxykinase gene were used to form chimeric gene constructs with the human growth hormone gene. These constructs were transfected into several renal and one liver cell line and the production of growth hormone (HGH) measured by immunoassay. Cyclic-AMP and glucocorticoid responsiveness of HGH production was observed in all cell lines. In two lines, the rat NRK52E renal epithelial line and the rat H4IIE hepatoma cell line, both capable of expressing PEPCK, lowering extracellular pH increased HGH production several fold. Comparison of hormone and pH effect on cells transfected with a thymidine kinase promoter-HGH chimera indicated that the PEPCK 5′ flanking region effects were specific. Thus, part of the pH responsiveness of the PEPCK gene in vivo may be attributed to properties of the 5′ flanking regions.