Novel insights into immunohistochemical analysis for diagnosing serous neoplasm of the pancreas: aquaporin 1, stereocilin, and transmembrane protein 255B.
Novel insights into immunohistochemical analysis for diagnosing serous neoplasm of the pancreas: aquaporin 1, stereocilin, and transmembrane protein 255B.
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对诊断胰腺浆液性肿瘤的免疫组织化学分析的新见解：水通道蛋白 1、立体青霉素和跨膜蛋白 255B。
DOI：
10.1111/his.14456
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发表时间：
2021
期刊：
影响因子：
6.4
通讯作者：
Hiraoka N.
中科院分区：
文献类型：
--
作者：
Watase C;Fuse M;Ino Y;Naito C;Hiraoka N.
胰腺浆液性（囊性）肿瘤（SCN）通常是良性的，只有少数病例建议手术治疗。为了避免不必要的手术，对SCN的准确诊断至关重要。在本研究中，我们的目的是确定新的免疫组化标记，以区分SCN从其他tumors.Methods和resultsWe比较了综合基因表达谱的SCN与正常胰腺和胰腺导管腺癌（PDAC）。我们选择了在SCN中上调，在PDAC中表达最低或未表达，并且具有适用于免疫组织化学的特异性和可用抗体的候选分子，然后分析了它们在各种肿瘤中的免疫组织化学表达。我们选择了水通道蛋白1（AQP1）、立体定向蛋白（STRC）、成纤维细胞生长因子受体3（FGFR 3）和跨膜蛋白255 B（TMEM 255 B），它们在79%、100%、100%和100%的SCN病例中在SCN细胞中弥漫表达。AQP1在其他肿瘤中不表达，除了20%的粘液性囊性肿瘤（MCNs）和19%的PDAC。STRC很少在MCN、神经内分泌肿瘤（NEN）和PDAC中表达。FGFR 3在31%的导管内乳头状粘液性肿瘤（IPMN）、50%的导管内嗜酸性乳头状肿瘤、40%的NEN、30%的腺泡细胞癌、40%的实性假乳头状肿瘤和52%的PDAC中表达。TMEM255 B在其他肿瘤中不表达，除了50%的MCN，80%的胃亚型IPMN和29%的PDAC。所有的抗原通常表达在一个小比例的细胞时，他们是积极的肿瘤以外的SCN.ConclusionsThese研究结果表明，AQP1和STRC，并可能TMEM255 B，可作为SCN标志物。
AimsSerous (cystic) neoplasm (SCN) of the pancreas is generally benign, and surgical treatment is recommended in only a limited number of cases. To avoid unnecessary surgery, an accurate diagnosis of SCN is essential. In the present study, we aimed to identify new immunohistochemical markers with which to distinguish SCN from other tumours.Methods and resultsWe compared the comprehensive gene expression profiles of SCN with those of normal pancreas and pancreatic ductal adenocarcinoma (PDAC). We selected the candidate molecules that were up‐regulated in SCN, were minimally expressed or unexpressed in PDAC, and had specific and available antibodies suitable for immunohistochemistry, and then analysed their immunohistochemical expression in various tumours. We selected aquaporin 1 (AQP1), stereocilin (STRC), fibroblast growth factor receptor 3 (FGFR3), and transmembrane protein 255B (TMEM255B), which were diffusely expressed in SCN cells in 79%, 100%, 100% and 100% of SCN cases. AQP1 was not expressed in other tumours, except in 20% of mucinous cystic neoplasms (MCNs) and 19% of PDACs. STRC was rarely expressed in MCNs, neuroendocrine neoplasms (NENs), and PDACs. FGFR3 was expressed in 31% of intraductal papillary mucinous neoplasms (IPMNs), 50% of intraductal oncocytic papillary neoplasms, 40% of NENs, 30% of acinar cell carcinomas, 40% of solid pseudopapillary neoplasms, and 52% of PDACs. TMEM255B was not expressed in the other tumours, except in 50% of MCNs, 80% of gastric‐subtype IPMNs, and 29% of PDACs. All antigens were usually expressed in a small proportion of cells when they were positive in tumours other than SCN.ConclusionsThese findings indicate that AQP1 and STRC, and potentially TMEM255B, may act as SCN markers.