Induced Beige Adipocytes Improved Fat Graft Retention by Promoting Adipogenesis and Angiogenesis
Induced Beige Adipocytes Improved Fat Graft Retention by Promoting Adipogenesis and Angiogenesis
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诱导米色脂肪细胞通过促进脂肪生成和血管生成改善脂肪移植保留
DOI：
10.1097/prs.0000000000008227
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发表时间：
2021-07
影响因子：
3.6
通讯作者：
Cai Junrong
中科院分区：
文献类型：
--
作者：
Xia Jing;Zhu Huidong;Zhu Shengqian;Ge Jinbo;Wang Zijue;Lu Feng;Liao Yunjun;Cai Junrong
文本中提供了补充数字内容。背景：脂肪移植常用于治疗软组织缺陷。然而，脂肪移植背后的基本生物学原理仍不完全清楚。脂肪移植后脂肪褐变成米色脂肪组织的证据已被揭示，但其在脂肪移植中的作用仍不清楚。方法：从人脂肪抽吸物中获得诱导的米色脂肪细胞和脂肪干细胞，并用绿色荧光蛋白标记。每只裸鼠注射 300 mg 人脂肪抽吸液，其中含有绿色荧光蛋白标记的脂肪干细胞、绿色荧光蛋白标记的诱导米色脂肪细胞或磷酸盐缓冲盐水。 1、4、8和12周后收获移植脂肪进行免疫组织化学和组织学检查。比较移植物保留、血管化和脂肪形成基因表达。结果：诱导7天后，脂肪细胞实现褐变，多房脂滴，线粒体增多，褐变基因表达上调。第 12 周时，注射诱导米色脂肪细胞后的脂肪移植保留率显着高于注射磷酸盐缓冲盐水后（46.0 ± 4.9% vs 31.0 ± 3.6%；p = 0.01），但注射诱导米色脂肪细胞和脂肪干细胞后相似（p > 0.05）。诱导的米色脂肪细胞重新变白为白色脂肪细胞，并显示过氧化物酶体增殖物激活受体-γ表达上调。诱导的米色脂肪细胞增强了血管生成，但在形成血管结构方面不活跃。结论：诱导米色脂肪细胞和脂肪干细胞在提高脂肪移植物保留率方面具有可比性。诱导的米色脂肪细胞以旁分泌方式促进血管生成，并且在脂肪移植后易于重新变白。
Supplemental Digital Content is available in the text. Background: Fat grafting is commonly used in treating soft-tissue defects. However, the basic biology behind fat grafting is still not fully understood. Evidence of adipose browning into beige adipose tissue after fat grafting was revealed, but its role in fat grafting remains unclear. Methods: Induced beige adipocytes and adipose-derived stem cells were obtained from human lipoaspirates and labeled with green fluorescent protein. Nude mice were each injected with 300 mg of human lipoaspirate containing green fluorescent protein–labeled adipose-derived stem cells, green fluorescent protein–labeled induced beige adipocytes, or phosphate-buffered saline. Grafted fat was harvested after 1, 4, 8, and 12 weeks for immunohistochemistry and histologic examination. Graft retention, vascularization, and adipogenic gene expression were compared. Results: After 7 days’ induction, adipocytes achieved browning with multilocular lipid droplets, increased mitochondria, and up-regulated browning gene expression. Fat graft retention rates at week 12 were significantly higher after injection of induced beige adipocytes than after injection of phosphate-buffered saline (46.0 ± 4.9 percent versus 31.0 ± 3.6 percent; p = 0.01), but were similar after injection of induced beige adipocytes and adipose-derived stem cells (p > 0.05). Induced beige adipocytes underwent rewhitening into white adipocytes and showed up-regulation of peroxisome proliferator-activated receptor-γ expression. Induced beige adipocytes enhanced angiogenesis, but were not active in forming vessel structures. Conclusions: Induced beige adipocytes and adipose-derived stem cells were comparable in improving fat graft retention rates. Induced beige adipocytes promote angiogenesis in a paracrine manner and are prone to rewhitening after fat grafting.
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DOI：
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发表时间：
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影响因子：
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通讯作者：
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影响因子：
29
作者：
Wang QA;Song A;Chen W;Schwalie PC;Zhang F;Vishvanath L;Jiang L;Ye R;Shao M;Tao C;Gupta RK;Deplancke B;Scherer PE
通讯作者：
Scherer PE
使用转染 VEGF 的脂肪干细胞提高人类自体脂肪移植的存活率
影响因子：
3.6
作者：
Lu, Feng;Li, Jie;Fu, Bingchuan
通讯作者：
Fu, Bingchuan
影响因子：
4.8
作者：
Merlin, Jon;Sato, Masaaki;Hutchinson, Dana S.
通讯作者：
Hutchinson, Dana S.
影响因子：
1.2
作者：
Asano, A;Kimura, K;Saito, M
通讯作者：
Saito, M