Molecular phylogeny of the tribe Bovini (Bovidae, Bovinae) and the taxonomic status of the Kouprey, Bos sauveli Urbain 1937
Molecular phylogeny of the tribe Bovini (Bovidae, Bovinae) and the taxonomic status of the Kouprey, Bos sauveli Urbain 1937
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DOI：
10.1016/j.ympev.2004.08.009
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发表时间：
2004-12-01
影响因子：
4.1
通讯作者：
Ropiquet, A
中科院分区：
文献类型：
--
作者：
Hassanin, A;Ropiquet, A
口鱼是印度支那半岛非常罕见的牛类物种，很长一段时间没有活标本被描述，这表明它可能已经灭绝。它在Bovini部落中的系统地位仍然混乱，因为对形态特征的分析导致了几个相互矛盾的假设。一些作者还提出，它可能是班腾与嘎尔、泽布或水牛杂交产生的杂交物种。在这里，我们进行了Bovini部落的分子系统学研究，以确定Kouprey的分类地位。从保存在MNHN标本中的正型标本中提取DNA。对包含Bovini族描述的所有分类多样性和2065个核苷酸特征的矩阵进行了系统发育分析，这些特征代表三个不同的标记，即乳铁蛋白的启动子和两个线粒体基因(细胞色素b和细胞色素c氧化酶亚基II)。结果表明，库普雷属于牛亚族，可分为三个不同的支系：第一类包括家牛、斑马和欧洲野牛；第二类包括牦牛和美洲野牛；第三类包括库普雷、班腾和高尔。所有关于口蹄兽起源的杂交假说都可以被拒绝，这证实了它是一个真正的野生物种。分子测年和生物地理推断表明，Kouprey在亚洲上更新世从班腾和嘎尔分离出来。此外，在细胞色素b基因中检测到了几个分子特征，从而可以对口鱼进行分子鉴定。我们提出了一个基于分子分类学方法的保护项目，以追踪印度支那的高波利鸟，以确定一些种群是否仍在野外生存。(C)2004 Elsevier Inc.保留所有权利。
The kouprey is a very rare bovid species of the Indochinese peninsula, and no living specimen has been described for a long time, suggesting that it is possibly extinct. Its systematic position within the tribe Bovini remains confused since the analyses of morphological characters have led to several conflicting hypotheses. Some authors have also suggested that it could be a hybrid species produced by the crossing of the banteng with gaur, zebu, or water buffalo. Here we performed a molecular phylogeny of the tribe Bovini to determine the taxonomic status of the kouprey. DNA was extracted from the holotype specimen preserved in the MNHN collections. Phylogenetic analyses were carried out on a matrix including all the taxonomic diversity described in the tribe Bovini, and 2065 nucleotide characters, representing three different markers, i.e., the promotor of the lactoferrin and two mitochondrial genes (cytochrome b and subunit II of the cytochrome c oxidase). The results show that the kouprey belongs to the subtribe Bovina, and that three different clades can be evidenced into this group: the first includes the domestic ox, zebu, and European bison; the second incorporates the yak and American bison; and the third contains the kouprey, banteng and gaur. All hypotheses involving hybridization for the origin of the kouprey can be rejected, confirming that it is a real wild species. Molecular datings and biogeographic inferences suggest that the kouprey diverged from banteng and gaur during the Plio-Pleistocene of Asia. In addition, several molecular signatures were detected in the cytochrome b gene, permitting a molecular identification of the kouprey. We propose a conservation project based on a molecular taxonomy approach for tracking the kouprey in Indochina in order to determine whether some populations still survive in the wild. (C) 2004 Elsevier Inc. All rights reserved.