Protective effect of Hypericum perforatum in zymosan-induced multiple organ dysfunction syndrome:: Relationship to its inhibitory effect on nitric oxide production and its peroxynitrite scavenging activity
Protective effect of Hypericum perforatum in zymosan-induced multiple organ dysfunction syndrome:: Relationship to its inhibitory effect on nitric oxide production and its peroxynitrite scavenging activity
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DOI：
10.1016/j.niox.2006.05.006
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发表时间：
2007-02-01
影响因子：
3.9
通讯作者：
Cuzzocrea, Salvatore
中科院分区：
文献类型：
--
作者：
Di Paola, Rosanna;Mazzon, Emanuela;Cuzzocrea, Salvatore
贯叶连翘是一种药用植物，含有多种多酚类化合物，即总黄酮和酚酸类化合物。鉴于多酚类化合物具有较高的抗氧化能力，我们研究了贯叶连翘提取物对酵母多糖(500 mg/kg)所致的多器官功能障碍综合征的影响。作为生理盐水中的悬浮液)。在给予酵母多糖和/或贯通灵芝提取物18h后，评估大鼠的器官衰竭和全身炎症反应，并监测12d(体重损失和死亡率)。小鼠腹腔注射贯叶连翘提取物(30 mg/kg，酵母多糖后1h和6h)可减轻酵母多糖引起的腹膜渗出和多形核细胞的迁移，减轻肺、肠、胰腺损伤和肾功能障碍，减少肺、肠髓过氧化物酶的升高。免疫组织化学检测诱导型一氧化氮合酶(INOS)、硝基酪氨酸和聚腺苷二磷酸核糖(PAR)在注射酵母多糖的小鼠肺和肠组织中呈阳性染色。经酵母多糖处理的小鼠组织切片中硝基酪氨酸、诱导型一氧化氮合酶和PAR的染色程度显著降低。总之，这项研究首次提供了证据，证明贯叶连翘提取物减轻酵母多糖诱导的小鼠多器官功能障碍综合征的程度。(C)2006 Elsevier Inc.保留所有权利。
Hypericum perforatum is a medicinal plant species containing many polyphenolic compounds, namely flavonoids and phenolic acids. Since polyphenolic compounds have high antioxidant potential, we have investigated the effects of H. perforatum extract on the development of multiple organ dysfunction syndrome caused by zymosan (500 mg/kg, administered i.p. as a suspension in saline) in mice. Organ failure and systemic inflammation in rats was assessed 18 h after administration of zymosan and/or H. perforatum extract and monitored for 12 days (for loss of body weight and mortality). Treatment of mice with H. perforatum extract (30 mg/kg i.p., 1 and 6 h after zymosan) attenuated the peritoneal exudation and the migration of polymorphonuclear cells caused by zymosan, pulmonary, intestinal and pancreatic injury, and renal dysfunction as well as the increase in myeloperoxidase in the lung and intestine. Immunohistochemical analysis for inducible nitric oxide synthase (iNOS), nitrotyrosine, and poly(ADP-ribose) (PAR) revealed positive staining in lung and intestine tissues obtained from zymosan-injected mice. The degree of staining for nitrotyrosine, iNOS, and PAR was markedly reduced in tissue sections obtained from zymosan-treated mice, which received H. perforatum extract. In conclusion, this study provides evidence, for the first time, that H. perforatum extract attenuates the degree of zymosan-induced multiple organ dysfunction syndrome in mice. (c) 2006 Elsevier Inc. All rights reserved.