Isolation and purification of bovine and canine prothrombin.
Isolation and purification of bovine and canine prothrombin.
复制标题
牛和犬凝血酶原的分离和纯化。
DOI：
10.1016/0304-4165(65)90484-8
复制
发表时间：
1965
期刊：
影响因子：
--
通讯作者：
J. R. Carter
中科院分区：
文献类型：
--
作者：
H. Moore;S. Lux;O. Malhotra;S. Bakerman;J. R. Carter
描述了适用于牛和犬的凝血酶原的分离和纯化程序。该制剂产率高，比活性高，即每毫克蛋白质有2400 -3000爱荷华州单位，并在分析超电泳和移动边界电泳中显示单一对称组分。由于这种高比活性的制剂不能最佳地活化BaCO 3吸附的血清，因此有必要修改两阶段凝血酶原测定方法以提供最佳的活化剂活性。
A procedure for isolation and purification of prothrombin, applicable to both the bovine and canine species is described. The preparations are of good yield and of very high specific activity,viz.2400–3000 Iowa units per mg of protein and show a single symmetric component in both the analytical ultracentrifuge and by moving-boundary electrophoresis. Since preparations of such high specific activity are not activated optimally BaCO3-adsorbed serum, it is necessary to modify the two-stage prothrombin assay method to provide optimal activator activity.