Molecular Studies on the Microbial Diversity Associated with Mining-Impacted Coeur d’Alene River Sediments
Molecular Studies on the Microbial Diversity Associated with Mining-Impacted Coeur d’Alene River Sediments
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DOI：
10.1007/s00248-008-9445-0
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发表时间：
2009-07
影响因子：
3.6
通讯作者：
Gurdeep Rastogi;R. Sani;B. Peyton;J. Moberly;T. Ginn
中科院分区：
文献类型：
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作者：
Gurdeep Rastogi;R. Sani;B. Peyton;J. Moberly;T. Ginn
采用一种与养殖无关的方法研究了从科达伦河(CDAR)采集的高度金属污染的沉积物样品中的原核生物多样性。通过构建RNA聚合酶β亚基(RpoB)基因文库，研究细菌群落结构。系统进化分析表明，75.8%的热B克隆与β-变形杆菌有关，其余24.2%与γ-变形杆菌有关。所有的系统类型都与以前报道的金属或有机污染物丰富的环境中的可培养谱系非常相似。在一个古生菌16S rRNA基因文库中，70%的克隆属于克伦考古纲，30%属于Eurya chaeota。大多数Eurya chaeota序列与甲烷腐烂目的乙酰碎屑谱系有关。Eurya chaeota中的一个单一系统模式与可栽培的Eurya chaeotal谱系没有关联，可能代表了新的分类单元。多样性指数表明，与古细菌相比，CDAR沉积物中细菌的多样性更高。通过X射线荧光光谱对沉积物的表征表明，沉积物中含有大量有毒金属。据我们所知，这是第一次对受采矿影响的CDAR沉积物中存在的原核生物多样性进行的独立于培养的调查。
The prokaryotic diversity associated with highly metal-contaminated sediment samples collected from the Coeur d’Alene River (CdAR) was investigated using a cultivation-independent approach. Bacterial community structure was studied by constructing an RNA polymerase beta subunit (rpoB) gene library. Phylogenetic analysis revealed that 75.8% of therpoBclones were associated with β-Proteobacteria while the remaining 24.2% were with γ-Proteobacteria. All phylotypes showed close similarity to previously reported cultivable lineages from metal or organic contaminant-rich environments. In an archaeal 16S rRNA gene library, 70% of the clones were affiliated to Crenarchaeota, while 30% belonged to Euryarchaeota. Most of the Euryarchaeota sequences were related to acetoclastic lineages belonging to Methanosarcinales. A single phylotype within the Euryarchaeota showed no association with cultivable euryarchaeotal lineages and might represent novel taxon. Diversity indices demonstrated greater diversity of Bacteria compared to Archaea in CdAR sediments. Sediment characterization by the X-ray fluorescence spectroscopy revealed high amount of toxic metals. To our knowledge, this is the first culture-independent survey on the prokaryotic diversity present in mining-impacted sediments of CdAR.