Continental-level population differentiation and environmental adaptation in the mushroom Suillus brevipes.
Continental-level population differentiation and environmental adaptation in the mushroom Suillus brevipes.
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DOI：
10.1111/mec.13892
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发表时间：
2017-04
影响因子：
4.9
通讯作者：
Bruns TD
中科院分区：
文献类型：
--
作者：
Branco S;Bi K;Liao HL;Gladieux P;Badouin H;Ellison CE;Nguyen NH;Vilgalys R;Peay KG;Taylor JW;Bruns TD
测序技术的最新进展使研究人员能够更好地解决大空间尺度上微生物环境适应所涉及的模式和机制。在这里，我们研究了短乳杆菌在大陆尺度上适应气候的基因组基础，短乳杆菌是一种与松树根部共生的外生菌根真菌。我们使用北美七个地区55个个体的基因组数据进行基因组扫描，以检测正选择的信号，并评估温度和降水是否与遗传分化有关。我们发现，短链球菌总体上表现出较强的种群分化，在加拿大种群中存在潜在的混合。该物种还表现出正选择的基因组特征，以及与不同的气候制度和非生物环境参数显著相关的基因组位置。这些基因组区域包括参与物质跨膜运输的基因和可能参与冷应激反应的解旋酶活性。我们的研究通过识别可能的适应基因，为进一步研究真菌适应的遗传基础提供了框架，从而揭示了真菌大规模的环境适应。
Recent advancements in sequencing technology allowed researchers to better address the patterns and mechanisms involved in microbial environmental adaptation at large spatial scales. Here we investigated the genomic basis of adaptation to climate at the continental scale in Suillus brevipes, an ectomycorrhizal fungus symbiotically associated with the roots of pine trees. We used genomic data from 55 individuals in seven locations across North America to perform genome scans to detect signatures of positive selection and assess whether temperature and precipitation were associated with genetic differentiation. We found that S. brevipes exhibited overall strong population differentiation, with potential admixture in Canadian populations. This species also displayed genomic signatures of positive selection as well as genomic sites significantly associated with distinct climatic regimes and abiotic environmental parameters. These genomic regions included genes involved in transmembrane transport of substances and helicase activity potentially involved in cold stress response. Our study sheds light on large-scale environmental adaptation in fungi by identifying putative adaptive genes and providing a framework to further investigate the genetic basis of fungal adaptation.