Purification and function of two analgesic and anti-inflammatory peptides from coelomic fluid of the earthworm, Eisenia foetida
Purification and function of two analgesic and anti-inflammatory peptides from coelomic fluid of the earthworm, Eisenia foetida
复制标题
蚯蚓体腔液中两种镇痛抗炎肽的纯化及其功能
DOI：
10.1016/j.peptides.2017.01.016
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发表时间：
2017-03-01
期刊：
影响因子：
3
通讯作者：
Yang, Xinwang
中科院分区：
文献类型：
--
作者：
Li, Chunlong;Chen, Mengrou;Yang, Xinwang
抗炎和镇痛化合物在药物和治疗护理中的潜在应用已经变得越来越受关注。我们纯化和表征了两种新的镇痛和抗炎肽，VQ-5和AQ-5，从体腔液的蚯蚓（蚯蚓）。它们的一级结构分别为VSSVQ和AMADQ。两种肽，特别是AQ-5，在持续性神经性疼痛和炎症的小鼠模型中表现出镇痛活性。AQ-5还抑制肿瘤坏死因子α和环氧合酶-2的产生。AQ-5抑制了参与镇痛和抗炎功能的丝裂原活化蛋白激酶信号通路。因此，这些肽，特别是AQ-5的镇痛和抗炎作用，证明了它们作为开发新型镇痛药物的候选物的潜力。(C)2017爱思唯尔公司All rights reserved.
The potential application of anti-inflammatory and analgesic compounds in medication and therapeutic care have become of increasing interest. We purified and characterized two novel analgesic and anti-inflammatory peptides, VQ-5 and AQ-5, from the coelomic fluid of the earthworm (Eisenia foetida). Their primary structures were determined as VSSVQ and AMADQ respectively. Both peptides, especially AQ-5, exhibited analgesic activity in mouse models of persistent neuropathic pain and inflammation. AQ-5 also inhibited tumor necrosis factor alpha and cyclooxygenase-2 production. The mitogen-activated protein kinase signaling pathway, which is involved in analgesic and anti-inflammatory functions, was inhibited by AQ-5. Thus, the analgesic and anti-inflammatory effects of these peptides, especially AQ-5, demonstrated their potential as candidates for the development of novel analgesic medicines. (C) 2017 Elsevier Inc. All rights reserved.