Visual input to the mouse lateral posterior and posterior thalamic nuclei: photoreceptive origins and retinotopic order.
Visual input to the mouse lateral posterior and posterior thalamic nuclei: photoreceptive origins and retinotopic order.
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DOI：
10.1113/jp271707
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发表时间：
2016-04-01
期刊：
影响因子：
--
通讯作者：
Brown TM
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文献类型：
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作者：
Allen AE;Procyk CA;Howarth M;Walmsley L;Brown TM
外侧后核和丘脑后核涉及视觉引导行为和对光的反射反应，包括依赖于黑视蛋白光感受的那些。在这里，我们调查的程度和基本特性的视觉诱发活动的小鼠后外侧和后丘脑。我们发现，这些区域的视网膜投射的一个子集来自表达黑视素的视网膜神经节细胞，并发现许多细胞在放电中表现出黑视素依赖性变化。我们还表明，这些区域的细胞亚群以各种方式整合来自双眼的信号，并且在外侧后丘脑内，视觉反应是视网膜定位有序的。除了外侧膝状体中的初级丘脑皮质视觉中继外，许多其他丘脑区域也参与视觉处理。因此，外侧丘脑后核（LP/枕）出现重要的各种功能，包括确定视觉显着性，视觉引导的行为，以及背侧部分的丘脑后核（Po），多感官处理相关的信息厌恶刺激。然而，尽管小鼠作为理解视觉系统组织的模型越来越重要，但目前我们对小鼠LP或Po细胞的基本视觉反应特性知之甚少。先前的建议认为黑视素光感受可能对这些核的某些功能很重要，我们首先采用特定的病毒示踪来显示视网膜投射到LP的子集来自表达黑视素的视网膜神经节细胞。接下来，我们使用多电极电生理学来证明LP和背侧Po细胞对简单视觉刺激表现出多种反应，包括两种不同的类别，它们表达黑视蛋白依赖性的放电变化（总共占我们记录的神经元的0.25%）。我们还表明，LP/Po细胞的亚群以各种方式整合来自两只眼睛的信号，并且在LP内，视觉反应是retinotopically有序的。我们的数据一起揭示了不同的人口的视觉反应神经元的LP和背Po的属性与这些核的一些既定的功能，并提出新的可能的路线，通过黑视蛋白光感受可以有助于反射光反应和/或高阶视觉处理。外侧后核和丘脑后核涉及视觉引导行为和对光的反射反应，包括依赖于黑视蛋白光感受的那些。在这里，我们调查的程度和基本特性的视觉诱发活动的小鼠后外侧和后丘脑。我们发现，这些区域的视网膜投射的一个子集来自表达黑视素的视网膜神经节细胞，并发现许多细胞在放电中表现出黑视素依赖性变化。我们还表明，这些区域的细胞亚群以各种方式整合来自双眼的信号，并且在外侧后丘脑内，视觉反应是视网膜定位有序的。
The lateral posterior and posterior thalamic nuclei have been implicated in aspects of visually guided behaviour and reflex responses to light, including those dependent on melanopsin photoreception. Here we investigated the extent and basic properties of visually evoked activity across the mouse lateral posterior and posterior thalamus. We show that a subset of retinal projections to these regions derive from melanopsin‐expressing retinal ganglion cells and find many cells that exhibit melanopsin‐dependent changes in firing. We also show that subsets of cells across these regions integrate signals from both eyes in various ways and that, within the lateral posterior thalamus, visual responses are retinotopically ordered. In addition to the primary thalamocortical visual relay in the lateral geniculate nuclei, a number of other thalamic regions contribute to aspects of visual processing. Thus, the lateral posterior thalamic nuclei (LP/pulvinar) appear important for various functions including determining visual saliency, visually guided behaviours and, alongside dorsal portions of the posterior thalamic nuclei (Po), multisensory processing of information related to aversive stimuli. However, despite the growing importance of mice as a model for understanding visual system organisation, at present we know very little about the basic visual response properties of cells in the mouse LP or Po. Prompted by earlier suggestions that melanopsin photoreception might be important for certain functions of these nuclei, we first employ specific viral tracing to show that a subset of retinal projections to the LP derive from melanopsin‐expressing retinal ganglion cells. We next use multielectrode electrophysiology to demonstrate that LP and dorsal Po cells exhibit a variety of responses to simple visual stimuli including two distinct classes that express melanopsin‐dependent changes in firing (together comprising ∼25% of neurons we recorded). We also show that subgroups of LP/Po cells integrate signals from both eyes in various ways and that, within the LP, visual responses are retinotopically ordered. Together our data reveal a diverse population of visually responsive neurons across the LP and dorsal Po whose properties align with some of the established functions of these nuclei and suggest new possible routes through which melanopsin photoreception could contribute to reflex light responses and/or higher order visual processing. The lateral posterior and posterior thalamic nuclei have been implicated in aspects of visually guided behaviour and reflex responses to light, including those dependent on melanopsin photoreception. Here we investigated the extent and basic properties of visually evoked activity across the mouse lateral posterior and posterior thalamus. We show that a subset of retinal projections to these regions derive from melanopsin‐expressing retinal ganglion cells and find many cells that exhibit melanopsin‐dependent changes in firing. We also show that subsets of cells across these regions integrate signals from both eyes in various ways and that, within the lateral posterior thalamus, visual responses are retinotopically ordered.