Characterization of a polysaccharide from Rosa davurica and inhibitory activity against neutrophil migration.
Characterization of a polysaccharide from Rosa davurica and inhibitory activity against neutrophil migration.
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DOI：
10.1016/j.ijbiomac.2016.04.062
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发表时间：
2016-08
影响因子：
8.2
通讯作者：
Haibin Tong;Guiquan Jiang;X. Guan;Hong Wu;Kangxing Song;Kuan Cheng;Xin Sun
中科院分区：
文献类型：
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作者：
Haibin Tong;Guiquan Jiang;X. Guan;Hong Wu;Kangxing Song;Kuan Cheng;Xin Sun
中性粒细胞从外周血快速募集到感染部位是炎症反应的关键步骤;然而，过度和不适当的募集可导致严重的组织损伤。因此，抑制它们的过度募集用于治疗炎症相关疾病是一种有前景的策略。本文从刺玫果中分离纯化了一种多糖（RDPA 1），并对其理化性质和对中性粒细胞迁移的抑制作用进行了研究。RDPA 1经热水提取、乙醇沉淀、DEAE-cellulose和SepharoseCL-6 B柱分离得到。RDPA 1可明显抑制人中性粒细胞的体外迁移，并影响时间推移显微镜观察到的细胞迁移行为，发现RDPA 1处理后的细胞迁移距离和平均迁移速度明显降低。此外，RDPA 1治疗还能减少腹膜炎小鼠体内的神经细胞浸润。流式细胞术和体外蛋白结合实验表明RDPA 1对β2整合素与ICAM-1的相互作用具有明显的阻断作用。总之，这些结果表明RDPA 1可以被认为是开发新型抗炎剂的潜在候选者。
The rapid recruitment of neutrophils from peripheral blood into infected sites is critical step for inflammatory responses; however, the excessive and improper recruitment can lead to serious tissue damages. Thus, it is a promising strategy to inhibit their excessive recruitment for treating inflammation-related disease. Here, we isolated a polysaccharide (RDPA1) fromRosa davurica, to evaluate its physicochemical property and inhibitory effects on neutrophil migration. RDPA1 was obtained by hot-water extraction, ethanol precipitation, and fractionated by DEAE-cellulose and Sepharose CL-6B columns. RDPA1 significantly inhibitedin vitromigration of human neutrophils evaluated by transwell chamber and impacted the migratory behavior observed by time-lapsed microscopy, we found the migrated distance and average velocity of RDPA1-treated cells were greatly reduced. In addition, RDPA1 treatment impairedin vivoneutrophil infiltration in the peritonitis mice. RDPA1 exhibited significant blocking capacity of the interaction between β2 integrins and ICAM-1 evaluated by flow cytometry andin vitroprotein binding assay. Together, these results suggest RDPA1 could be considered as a potential candidate for developing a novel anti-inflammatory agent.