GROWTH AND PHYSIOLOGICAL FUNCTIONS OF VASCULAR ENDOTHELIAL-CELLS IN A NEW SERUM-FREE MEDIUM (SFM)
GROWTH AND PHYSIOLOGICAL FUNCTIONS OF VASCULAR ENDOTHELIAL-CELLS IN A NEW SERUM-FREE MEDIUM (SFM)
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DOI：
10.1006/excr.1993.1149
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发表时间：
1993-06-01
影响因子：
3.7
通讯作者：
WEISS, S
中科院分区：
文献类型：
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作者：
GORFIEN, S;SPECTOR, A;WEISS, S
体外生长的内皮细胞被广泛用于血管生理学和病理学的研究。通常在内皮细胞培养中使用的高水平血清补充可能会在实验设计或结果解释中产生问题。一种无血清培养基（SFM）被设计用于培养非人血管内皮细胞。在牛、猪、羊和犬的大血管内皮细胞中，各种生理标记物在这种培养基中生长和保留。所有被试物种的细胞都吸收了二乙酰化的LDL。在牛和犬细胞的培养中证实了因子viii相关抗原。牛和猪的细胞被证明保留了产生前列环素的能力。进一步补充各种含脂添加剂的SFM未能刺激生长或产生前列环素。测量了牛、猪和犬内皮细胞产生的PDGF，发现其浓度低于血清补充培养。在SFM中生长的所有细胞都表现出组织型的“鹅卵石”形态，通常与培养的内皮细胞相关。在SFM中添加氢化可的松、牛垂体提取物和表皮生长因子，可实现人脐静脉内皮细胞的短期生长（≤4代），但未对人内皮细胞的培养进行优化。
Endothelial cells grownin vitroare widely used for the study of vascular physiology and pathology. The high levels of serum supplementation normally used in endothelial cell culture may create problems in experimental design or in interpretation of results. A serum-free medium (SFM) has been designed specifically for culture of nonhuman vascular endothelial cells. Growth as well as retention of various physiological markers in this medium was demonstrated in large-vessel endothelial cells from bovine, porcine, ovine, and canine species. Cells from all species tested took up DiI-acetylated LDL. Factor VIII-related antigen was demonstrated in cultures of bovine and canine cells. Bovine and porcine cells were shown to have retained the ability to produce prostacyclin. Further supplementation of the SFM with various lipid-containing additives failed to stimulate either growth or prostacyclin production. PDGF produced by bovine, porcine, and canine endothelial cells was measured and found to be present at lower concentration than in serum supplemented cultures. All cells grown in SFM exhibited the histiotypic "cobblestone" morphology normally associated with cultured endothelial cells. While not optimized for culture of human endothelial cells, short-term growth ≤ 4 passages) of human umbilical vein endothelial cells was achieved by supplementing the SFM with hydrocortisone, bovine pituitary extract, and epidermal growth factor.