Growth factor release from thermally reversible tissue culture substrates.
Growth factor release from thermally reversible tissue culture substrates.
复制标题
生长因子从热可逆组织培养基质中释放。
DOI：
10.1016/s0168-3659(98)00042-x
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
WanKim,S
中科院分区：
文献类型：
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作者：
vonRecum,H;Okano,T;WanKim,S
将热可逆聚（N-异丙基丙烯酰胺）（PIPAAm）共价接枝到组织培养皿上，以允许在温度从生理温度变化到室温时培养细胞脱离。此外，接枝的聚合物基质用于捕获生物分子，例如生长因子，以在细胞培养物中通过扩散早期释放，或保持捕获并可逆地暴露于细胞受体。用模型蛋白胰蛋白酶和胰岛素的实验表明，负载和释放的量取决于PIPAAm接枝密度。用2.5 μg/cm 2 PIPAAm接枝的培养皿在4小时内释放的模型蛋白比用1.8 μg/cm 2接枝的培养皿多约4倍。这种体外药物递送系统可用于在细胞培养早期通过提供高局部浓度而不提供高本体浓度来将因子递送至细胞的基底侧。人视网膜色素上皮细胞培养物在胰岛素加载培养皿上的生长速率高于含有类似原液浓度的对照。这些培养物保留了单独或作为汇合片从加载表面分离的能力。
Thermally reversible poly(N-isopropylacrylamide) (PIPAAm) was covalently grafted onto tissue culture dishes to allow detachment of cultured cells upon temperature change from physiological to room temperature. In addition the grafted polymer matrix was used to entrap biomolecules such as growth factors either to be released by diffusion early in cell cultures, or remain entrapped and be reversibly exposed to cell receptors. Experiments with model proteins trypsin and insulin show that amount loaded and released depends upon the PIPAAm grafting density. Dishes grafted with 2.5 μg/cm2PIPAAm released approximately four times more model protein over 4 h than dishes grafted with 1.8 μg/cm2. This in vitro drug delivery system can be used to deliver factors to the basal side of cells early in cell culture by providing high local concentrations without high bulk concentration. Cultures of human retinal pigmented epithelium showed higher growth rate on insulin loaded dishes than on controls containing a similar bulk solution concentration. These cultures retained the ability to detach singly or as confluent sheets from the loaded surfaces.