Modulation of the humoral immune response by antibody-mediated antigen targeting to complement receptors and Fc receptors.
Modulation of the humoral immune response by antibody-mediated antigen targeting to complement receptors and Fc receptors.
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通过抗体介导的抗原靶向补体受体和 Fc 受体来调节体液免疫反应。
DOI：
10.4049/jimmunol.162.6.3125
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发表时间：
1999
影响因子：
4.4
通讯作者：
Wouter L. W. Hazenbos
中科院分区：
文献类型：
--
作者：
Dana C. Baiu;J. Prechl;A. Tchorbanov;Hector Molina;A. Erdei;A. Sulica;Peter J. A. Capel;Wouter L. W. Hazenbos
在持续的免疫应答期间，形成由Ag、补体因子和Ig组成的免疫复合物，其可与补体受体（CR）和IgG Fc受体（Fc γ R）相互作用。使用与大鼠抗小鼠CR 1/2 mAb 7 G6的完整IgG化学偶联的OVA研究了CR 1/2和Fc γ R在免疫应答调节中的作用。使用鼠B细胞淋巴瘤IIA 1.6的FACS分析证实了7 G6-OVA缀合物识别CR 1/2。将IIA 1.6细胞与7 G6-OVA一起孵育在体外触发了酪氨酸磷酸化和向OVA特异性T细胞的Ag呈递。以每只小鼠1微克腹腔注射的最小剂量用7 G6-OVA免疫小鼠显著增强了抗-OVA IG应答，其主要是IgG 1同种型亚类。7 G6-OVA不增强CR 1/2缺陷小鼠中的抗OVA应答。与单独的OVA诱导的抗-OVA应答相比，与同种型对照Ab偶联的OVA诱导的抗-OVA应答显著更低，表明通过Ab的Fc部分与B细胞上的抑制性Fc γ RIIb之间的相互作用的抑制。这一发现得到以下观察结果的支持：用7 G6-OVA孵育的IIA 1.6细胞在用Fc γ RIIb转染后丧失呈递Ag的能力。总之，类似于天然免疫复合物的7 G6缀合的OVA诱导增强的抗OVA免疫应答，其涉及至少CR 1/2介导的刺激并且可被Fc γ RIIb部分抑制。
During an ongoing immune response, immune complexes, composed of Ag, complement factors, and Igs, are formed that can interact with complement receptors (CRs) and IgG Fc receptors (Fc gamma R). The role of CR1/2 and Fc gamma R in the regulation of the immune response was investigated using OVA that was chemically conjugated to whole IgG of the rat anti-mouse CR1/2 mAb 7G6. FACS analysis using the murine B cell lymphoma IIA1.6 confirmed that the 7G6-OVA conjugate recognized CR1/2. Incubating IIA1.6 cells with 7G6-OVA triggered tyrosine phosphorylation and Ag presentation to OVA-specific T cells in vitro. Immunizing mice with 7G6-OVA at a minimal dose of 1 microgram i.p. per mouse markedly enhanced the anti-OVA Ig response, which was primarily of the IgG1 isotype subclass. The 7G6-OVA did not enhance the anti-OVA response in CR1/2-deficient mice. OVA coupled to an isotype control Ab induced a considerably lower anti-OVA response compared with that induced by OVA alone, suggesting inhibition by interaction between the Fc part of the Ab and the inhibitory Fc gamma RIIb on B cells. This findings was supported by the observation that IIA1.6 cells which were incubated with 7G6-OVA lost the ability to present Ag upon transfection with Fc gamma RIIb. In sum, 7G6-conjugated OVA, resembling a natural immune complex, induces an enhanced anti-OVA immune response that involves at least CR1/2-mediated stimulation and that may be partially suppressed by Fc gamma RIIb.
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影响因子：
4.4
作者：
Y. Kozono;Y. Kozono;R. Abe;H. Kozono;Robert G. Kelly;T. Azuma;V. Holers
通讯作者：
Y. Kozono;Y. Kozono;R. Abe;H. Kozono;Robert G. Kelly;T. Azuma;V. Holers
当抗原受体与 CD21 (CR2) 共连接时，通过抗原受体复合物激活人 B 细胞的亲和力阈值会降低。
DOI：
--
发表时间：
1997
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Mongini,PK;Vilensky,MA;Highet,PF;Inman,JK
通讯作者：
Inman,JK
通过经典途径补体调节 B 细胞对 T 依赖性抗原的反应。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fischer,MB;Ma,M;Goerg,S;Zhou,X;Xia,J;Finco,O;Han,S;Kelsoe,G;Howard,RG;Rothstein,TL;Kremmer,E;Rosen,FS;Carroll,MC
通讯作者：
Carroll,MC
小鼠 B 细胞肿瘤 A20/2J 的不同表型变体是通过 L3T4 阳性、I-A 限制性 T 细胞克隆的抗原和丝裂原触发的细胞毒性来选择的。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Jones,B;Tite,JP;JanewayJr,CA
通讯作者：
JanewayJr,CA
小鼠补体受体 1 和 2 的调节可抑制体内抗体反应。
DOI：
--
发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thyphronitis,G;Kinoshita,T;Inoue,K;Schweinle,JE;Tsokos,GC;Metcalf,ES;Finkelman,FD;Balow,JE
通讯作者：
Balow,JE