The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.
The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.
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DOI：
10.1016/s1534-5807(03)00396-4
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发表时间：
2004
影响因子：
11.8
通讯作者：
Gerlinde Reim;Takamasa Mizoguchi;D. Stainier;Y. Kikuchi;M. Brand
中科院分区：
文献类型：
--
作者：
Gerlinde Reim;Takamasa Mizoguchi;D. Stainier;Y. Kikuchi;M. Brand
原肠脊椎动物胚胎发育三个胚层：外胚层、中胚层和内胚层。斑马鱼内胚层分化始于Casanova(CAS)对Sox17的激活。我们报道了spg(pou2/Oct4)是内胚层形成所必需的。缺乏母体和受精卵功能的胚胎(MZspg)缺乏内胚层前体。细胞移植表明，在早期的内胚层前体中有spgts，而将CasmRNA注射到MZspg胚胎中并不能恢复内胚层的发育，spg和Castogogether是激活内胚层发育的必要条件和充分条件，并刺激asox17启动子-荧光素酶报告基因的表达。内胚层和中胚层起源于共同的来源，即中胚层。我们认为，SPG和Cas将中内胚层前体与内胚层命运联系起来。SPG和CASE对内胚层形成的共同控制表明，内胚层可能是一个具有不同遗传控制的组织单位，从而为后生动物发育中的胚层概念提供了遗传支持。
The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation ofsox17bycasanova(cas). We report thatspg(pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygoticspgfunction (MZspg) lack endodermal precursors. Cell transplantations show thatspgacts in early endodermal precursors, andcasmRNA-injection into MZspgembryos does not restore endoderm development.spgandcastogether are both necessary and sufficient to activate endoderm development, and stimulate expression of asox17promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation byspgandcassuggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.