Characterization of nitric oxide-inducing lipid A derived from Mesorhizobium loti lipopolysaccharide.
Characterization of nitric oxide-inducing lipid A derived from Mesorhizobium loti lipopolysaccharide.
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DOI：
10.1264/jsme2.me12103
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发表时间：
2012
影响因子：
2.2
通讯作者：
Uchiumi T
中科院分区：
文献类型：
--
作者：
Hashimoto M;Tanishita Y;Suda Y;Murakami E;Nagata M;Kucho K;Abe M;Uchiumi T
百脉根中根瘤菌是根瘤菌的一员，与几种百脉根形成固氮共生体。最近，有报道称，M.百脉根细菌细胞及其脂多糖（LPS）制剂能瞬时诱导百脉根产生一氧化氮（NO）。- 是的我们随后发现，多糖和脂质A部分负责这种NO诱导。本研究中，我们阐明了M. loti脂质A，并表征其响应于结构修饰的NO诱导活性。M.用肼或氢氟酸水溶液分别部分水解loti LPS以获得O-脱酰化或脱磷酸化LPS。对未处理和处理的LPS级分进行弱酸水解以获得脂质A级分。M.通过化学成分分析、MALDI-TOF-MS和NMR谱，鉴定loti lipid A为β-GlcNNα-GlcNNα-GalA，其中β-GlcNN的2和3位被3-酰氧基脂肪酰胺取代，α-GlcNN的2和3位被3OH脂肪酰胺取代。脂质A的部分水解似乎降低其NO诱导活性。这些结果提示L.发根细胞识别脂质A结构作为控制NO产生的手段。
Mesorhizobium loti is a member of the rhizobia and forms nitrogen-fixing symbioses with several Lotus species. Recently, it was reported that M. loti bacterial cells and their lipopolysaccharide (LPS) preparations transiently induced nitric oxide (NO) production in the roots of L. japonicus. We subsequently found that polysaccharides and the lipid A moiety were responsible for this NO induction. In this study, we elucidated the chemical structure of M. loti lipid A and characterized its NO-inducing activity in response to structural modifications. M. loti LPS were partially hydrolyzed with hydrazine or aqueous hydrofluoric acid to obtain O-deacylated or dephosphorylated LPS, respectively. The untreated and treated LPS fractions were subjected to weak acid hydrolysis to obtain lipid A fractions. The chemical structure of M. loti lipid A was elucidated by chemical composition analysis, MALDI-TOF-MS, and NMR spectra to be P-4-β-GlcNNα-GlcNNα-GalA, in which positions 2 and 3 of β-GlcNN are substituted for 3-acyloxy-fatty amides, and positions 2 and 3 of α-GlcNN are substituted for 3OH-fatty amides. The partial hydrolysis of lipid A appeared to reduce its NO-inducing activity. These results suggest that L. japonicus root cells recognize the lipid A structure as a means of controlling NO production.
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DOI：
10.1084/jem.189.11.1777
发表时间：
1999-06-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Shimazu R;Akashi S;Ogata H;Nagai Y;Fukudome K;Miyake K;Kimoto M
通讯作者：
Kimoto M
影响因子：
3.2
作者：
Ardissone, Silvia;Kobayashi, Hajime;Deakin, William J.
通讯作者：
Deakin, William J.
影响因子：
3.5
作者：
Vedam, V;Haynes, JG;Sherrier, DJ
通讯作者：
Sherrier, DJ
影响因子：
4.8
作者：
Hinckley, MB;Reynolds, CM;Raetz, CRH
通讯作者：
Raetz, CRH
影响因子：
30.5
作者：
Hajjar, AM;Ernst, RK;Miller, SI
通讯作者：
Miller, SI