Effects of exercise on depressive behavior and striatal levels of norepinephrine, serotonin and their metabolites in sleep-deprived mice
Effects of exercise on depressive behavior and striatal levels of norepinephrine, serotonin and their metabolites in sleep-deprived mice
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DOI：
10.1016/j.bbr.2017.05.062
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发表时间：
2017-08-14
影响因子：
2.7
通讯作者：
Sales de Bruin, Veralice Meireles
中科院分区：
文献类型：
--
作者：
da Costa Daniele, Thiago Medeiros;Carvalhedo de Bruin, Pedro Felipe;Sales de Bruin, Veralice Meireles
运动是治疗抑郁行为、睡眠/觉醒异常、认知和运动功能障碍的一种很有前途的方法。相反，睡眠不足会损害情绪，认知和功能表现。本研究的目的是评价运动对6 h完全睡眠剥夺（6 h-TSD）和72 h快速眼动（REM）睡眠剥夺（72 h-REMSD）小鼠焦虑和抑郁行为以及纹状体去甲肾上腺素（NE）、5-羟色胺（5-HT）及其代谢产物水平的影响。实验组为：（1）通过温和处理进行6 h-TSD的小鼠;（2）通过花盆法进行72 h-REMSD的小鼠;（3）运动（踏车8周）;（4）运动后进行6 h-TSD;（5）运动后进行72 h-REMSD;（6）对照（饲养笼）。行为学测试包括高架十字迷宫和尾部悬吊。NE，5-羟色胺及其代谢产物测定纹状体使用高效液相色谱法（HPLC）。睡眠剥夺增加了抑郁行为（在尾部悬吊试验中的固定时间），先前的运动阻碍了它。睡眠剥夺增加了纹状体NE，先前的运动减少了它。运动只与较高的血清素水平有关。此外，运动减少了与睡眠剥夺有关的血清素周转。简而言之，先前的运动预防了抑郁行为，降低了纹状体高NE水平和5-羟色胺周转。目前的研究结果证实了运动对睡眠剥夺相关的行为和神经化学变化的影响。这些发现为理解运动机制提供了新的途径。
Exercise is a promising adjunctive therapy for depressive behavior, sleep/wake abnormalities, cognition and motor dysfunction. Conversely, sleep deprivation impairs mood, cognition and functional performance. The objective of this study is to evaluate the effects of exercise on anxiety and depressive behavior and striatal levels of norepinephrine (NE), serotonin and its metabolites in mice submitted to 6 h of total sleep deprivation (6h-TSD) and 72 h of Rapid Eye Movement (REM) sleep deprivation (72h-REMSD). Experimental groups were: (1) mice submitted to 6h-TSD by gentle handling; (2) mice submitted to 72h-REMSD by the flower pot method; (3) exercise (treadmill for 8 weeks); (4) exercise followed by 6h-TSD; (5) exercise followed by 72h-REMSD; (6) control (home cage). Behavioral tests included the Elevated Plus Maze and tail-suspension. NE, serotonin and its metabolites were determined in the striatum using high-performance liquid chromatography (HPLC). Sleep deprivation increased depressive behavior (time of immobilization in the tail-suspension test) and previous exercise hindered it. Sleep deprivation increased striatal NE and previous exercise reduced it. Exercise only was associated with higher levels of serotonin. Furthermore, exercise reduced serotonin turnover associated with sleep deprivation. In brief, previous exercise prevented depressive behavior and reduced striatal high NE levels and serotonin turnover. The present findings confirm the effects of exercise on behavior and neurochemical alterations associated with sleep deprivation. These findings provide new avenues for understanding the mechanisms of exercise.