The termini of the chlorella virus PBCV-1 genome are identical 2.2-kbp inverted repeats.

The termini of the chlorella virus PBCV-1 genome are identical 2.2-kbp inverted repeats.
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DOI:
10.1016/0042-6822(91)90089-t
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发表时间:
1991-02
期刊:
影响因子:
3.7
通讯作者:
Peter Strasser;Yanping Zhang;Jan Rohozinski;J. L. Etten
Peter Strasser;Yanping Zhang;Jan Rohozinski;J. L. Etten
中科院分区:
医学3区
文献类型:
--
作者:
Peter Strasser;Yanping Zhang;Jan Rohozinski;J. L. Etten

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Chlorellavirus PBCV-1基因组是一个333 kbp的线性双链DNA分子,具有共价闭合的发夹末端。末端(减去发夹)是至少2185个碱基的相同反向重复序列,之后序列发散。反向重复序列包含两个小的潜在开放阅读框架和几个直接重复序列。然而,在PBCV-1复制过程中,开放阅读框和反向重复序列的其余部分都不转录。在36个检测的Chlorellavirus DNA中,有29个与PBCV-1末端片段杂交。
TheChlorellavirus PBCV-1 genome is a linear nonpermuted 333-kbp dsDNA molecule with covalently closed hairpin termini. The termini (minus the hairpin) are identical inverted repeats of at least 2185 bases after which the sequence diverges. The inverted repeats contain two small potential open reading frames and several direct repeats. However, neither the open reading frames nor the remainder of the inverted repeats are transcribed during PBCV-1 replication. Twenty-nine otherChlorellavirus DNAs, of 36 tested, hybridized to the PBCV-1 terminal fragments.