Developmental changes in the responses of rat chromaffin cells to neuronotrophic and neurite-promoting factors.
Developmental changes in the responses of rat chromaffin cells to neuronotrophic and neurite-promoting factors.
复制标题
大鼠嗜铬细胞对神经营养和神经突促进因子反应的发育变化。
DOI：
10.1016/0012-1606(85)90495-6
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Varon,S
中科院分区：
文献类型：
--
作者：
Unsicker,K;Skaper,SD;Varon,S
本研究描述了从出生后1天（D1）至100天（D100）龄的大鼠中获得的培养肾上腺髓质（嗜铬）细胞对神经生长因子（NGF）、鸡眼睫状神经营养因子（CNTF）和层粘连蛋白的反应的存活和神经突生长行为。在缺乏营养因子的情况下，培养的嗜铬细胞的4天存活率（相对于2小时时附着的细胞数）从D1时的三分之一增加到D8时的40%和D16及更老阶段的90-100%。在饱和浓度的NGF增加细胞存活在D8的90%，但未能支持所有的嗜铬细胞在2 hr. In相比之下，CNTF支持所有细胞的生存在D8。在D1，NGF和CNTF在4天的培养期间对存活率只有非常小的影响，尽管这两个因素在8天后明显增强了存活细胞的数量。无论是NGF还是CNTF都能引起大鼠嗜铬细胞的轴突生长，在D1和D8时约为15-20%，随后在D30时降至约5-8%，在D100时几乎为零。在最后一个年龄段，两种因素共同作用明显诱发了神经突。这种增强作用的NGF和CNTF也被认为是在出生后的早期年龄。层粘连蛋白并没有影响神经突起的生长在D30的营养因子的情况下，已经描述了D8大鼠嗜铬细胞。然而，在NGF的存在下，它在4天的培养期间增加了神经突长度和分支，甚至在以后的培养时间增强了神经突的募集。这些数据表明，大鼠嗜铬细胞经历了与年龄相关的变化，他们对NGF和CNTF的反应和层粘连蛋白调节他们的神经突起生长的行为在营养因子的存在。
This study describes the survival and neurite outgrowth behaviors of cultured adrenal medullary (chromaffin) cells obtained from postnatal rats 1 day (D1) to 100 days (D100) old in response to nerve growth factor (NGF), chick eye ciliary neuronotrophic factor (CNTF), and laminin. In the absence of trophic factors the 4-day survival of cultured chromaffin cells (relative to the number of cells attached at 2 hr) increased from one-third of the cells at D1 to 40% at D8 and 90–100% at D16 and older stages. At saturating concentrations NGF increased cell survival at D8 by 90%, but failed to support all chromaffin cells present at 2 hr. In contrast, CNTF supported the survival of all cells at D8. At D1 NGF and CNTF had only a very small effect on survival during the 4-day culture period, although both factors clearly enhanced the numbers of surviving cells after 8 days. Either NGF or CNTF also elicited neurite outgrowth from rat chromaffin cells, which amounted to approximately 15–20% at D1 and D8 and subsequently decreased to about 5–8% at D30 and virtually zero at D100. At this last age both factors applied together clearly elicited neurites. Such a potentiating effect of NGF and CNTF was also seen at earlier postnatal ages. Laminin did not affect neurite growth at D30 in the absence of trophic factors, as already described for D8 rat chromaffin cells. In the presence of NGF, however, it increased neurite length and branching during a 4-day culture period and even enhanced neurite recruitment at later culture times. These data suggest that rat chromaffin cells undergo age-related changes in their responses to NGF and CNTF and that laminin modulates their neurite outgrowth behaviors in the presence of trophic factors.