Transcriptional analysis of sex pheromone biosynthesis signal genes in Bombyx mori
Transcriptional analysis of sex pheromone biosynthesis signal genes in Bombyx mori
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DOI：
10.1111/j.1744-7917.2011.01469.x
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发表时间：
2012-12-01
期刊：
影响因子：
4
通讯作者：
Yin, Xin-Ming
中科院分区：
文献类型：
--
作者：
An, Shi-Heng;Du, Meng-Fang;Yin, Xin-Ming
研究了六种性信息素合成信号基因，包括酰基辅酶A（酰基辅酶A）去饱和酶（desat1）、脂肪酰基还原酶（FAR）、信息素生物合成激活神经肽受体（PBANR）、脂肪酸转运蛋白（FATP）、酰基辅酶A结合蛋白（ACBP）和存储操纵通道蛋白（Orai1A） 其转录调控。这些转录物在不同发育阶段（96 至 48 小时）的表达谱表明，这些基因以年龄依赖性方式表达。尽管断头，这些基因的转录本仍继续增加，并且与正常发育的雌性相比，断头显着抑制了它们的表达。使用保幼激素（JH）类似物甲氧普林进行的进一步实验表明，JH并不是这些基因表达的关键抑制因素，并且发现交配显着抑制这些标记基因的表达。总之，该结果为理解性信息素合成机制提供了参考。
Six sex pheromone synthesis signal genes, including acyl coenzyme A (acyl-CoA) desaturase (desat1), fatty acyl reductase (FAR), pheromone biosynthesis activating neuropeptide receptor (PBANR), fatty acid transport protein (FATP), acyl-CoA binding protein (ACBP) and store-operated channel protein (Orai1A), were studied for their transcriptional regulations. The expression profiles of these transcripts at different developmental stages (from 96 to 48 h) revealed that the genes are expressed in an age-dependent manner. The transcripts of these genes continued to increase despite decapitation, and compared with normally developmental females, decapitation significantly inhibited their expression. Further experiments with a methoprene, a juvenile hormone (JH) analogue, challenge showed that JH was not a key inhibiting factor in the expression of these genes, and mating was found to significantly inhibit the expression of these marker genes. Altogether, the results provide a reference for understanding the mechanism of sex pheromone synthesis.