Transcription of the Epstein-Barr virus genome in productively infected cells.
Transcription of the Epstein-Barr virus genome in productively infected cells.
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EB 病毒基因组在有效感染细胞中的转录。
DOI：
10.1016/0042-6822(83)90286-6
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Nonoyama,M
中科院分区：
文献类型：
--
作者：
Shin,S;Tanaka,A;Nonoyama,M
研究了EB病毒基因组在生产性感染的B 95 -8细胞中的转录。从细胞质病毒poly（A）RNA制备的cDNA与28个不同克隆的EBV DNA的BamHI片段的斑点杂交表明，在对照和TPA处理的细胞中，病毒基因组转录的模式相似。然而，三个片段，BamHI I，R和Z，似乎是新诱导TPA处理的细胞，因为没有显着水平的转录这些片段在对照细胞中观察到。相反，即使在用TPA处理的细胞中，也没有检测到来自片段BamHI P、a和B的转录。用琼脂糖凝胶电泳和Southern印迹杂交法测定病毒特异性RNA的大小。68胞质多聚（A）RNA的物种，从0.1到2.8兆道尔顿的大小不等，通过这种程序进行检测。
Transcription of the genome of Epstein-Barr virus in productively infected B95-8 cells was studied. Dot blot hybridization of cDNA prepared from cytoplasmic viral poly(A)RNA with 28 different cloned BamHI fragments of EBV DNA indicated that the patterns of viral genome transcription were similar in control and TPA-treated cells. However, three fragments,BamHI I, R, and Z, appeared to be newly induced in TPA-treated cells, as no significant level of transcription of these fragments was observed in control cells. In contrast, there was no detectable transcription from fragmentsBamHI P, a, and b even in cells treated with TPA. The size of virus-specific RNA was determined by agarose gel electrophoresis followed by Southern blot hybridization using radioactiveBamHI cloned EBV DNA fragments as probes. Sixty-eight cytoplasmic poly(A)RNA species, ranging in size from 0.1 to 2.8 megadaltons, were detected by this procedure.