The Inheritance of Nitrogen and Phosphorus Content in Barley Analysed by Genetic Markers

The Inheritance of Nitrogen and Phosphorus Content in Barley Analysed by Genetic Markers
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遗传标记分析大麦氮、磷含量的遗传

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发表时间:
2004
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通讯作者:
J. Jensen
J. Jensen
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作者:
B. Kjær;J. Jensen

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对79个F1代染色体加倍的大麦DH系进行了氮、磷含量的数量性状位点(QTL)分析。在田间试验中,记录了施氮和不施氮的情况下的产量以及籽粒和秸秆中的氮和磷浓度。 利用85位点连锁图对记录性状和由记录性状衍生的农艺性状的QTL定位进行了估计。除子粒和秸秆全氮量外,其余性状均存在极显著的QTL效应。不同性状的QTL主要聚集在第2染色体上的两个区域。位于第2染色体上V位点(二棱/六棱)附近的QTL可能是由V位点的多效性引起的。1个或2个QTL对籽粒和秸秆中氮、磷浓度的影响可解释DH系间变异的15 ~ 45%。第2染色体上的位点v与第2和第4染色体上的位点对籽粒和秸秆中氮、磷浓度存在互作效应。在二棱和六棱DH系中,秸秆和籽粒中N、P含量的变异很大程度上由不同的QTL所解释。
Quantitative trait loci (QTLs) for nitrogen and phosphorus content in barley were examined in 79 chromosome-doubled haploid (DH) lines derived from the F1-generation. Yield and concentration of nitrogen and phosphorus in grain and straw were recorded in a field trial with and without N-fertiliser application. The position of QTLs for recorded traits and for agronomic traits derived from the recorded traits were estimated using an 85-point linkage map. Highly significant QTLs effects were found for all traits except for the total quantity of nitrogen in grain and straw. QTLs for different traits were clustered especially in two regions on chromosome 2. The QTLs in the region around locus v (two-rowed/six-rowed) on chromosome 2 may be caused by pleiotropic effects of the locus v. One or two QTLs for nitrogen and phosphorus concentrations in grain and straw explained from 15 to 45% of the variation among DH lines. Interactions were found between locus v on chromosome 2 and loci on chromosomes 2 and 4 for nitrogen and phosphorus concentrations in grain and straw. Within the two-rowed and six-rowed DH lines a large part of the variation for N and P concentrations in straw and grain was explained by different QTLs.