Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells
Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells
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DOI：
10.1016/j.bbrc.2015.08.105
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发表时间：
2015-10-23
影响因子：
3.1
通讯作者：
Roh, Sang-gun
中科院分区：
文献类型：
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作者：
Suzuki, Yutaka;Haga, Satoshi;Roh, Sang-gun
Chemerin 是脂肪细胞和肝细胞产生的趋化细胞因子（趋化因子）；它调节胰岛素敏感性和脂肪细胞分化。本研究的目的是研究凯莫瑞对牛乳腺上皮细胞系 (MAC-T) 中泌乳相关基因表达和凯莫瑞信号调节因子的影响。在培养的 MAC-T 细胞中检测到两种类型的凯莫瑞受体：趋化因子样受体 1 (CMKLR1) 和趋化因子（C-C 基序）受体样 2 (CCRL2)，但未检测到凯莫瑞。 G 蛋白偶联受体 1 (GPR1) 是凯莫瑞的另一种受体，在 MAC-T 细胞中检测不到。 Chemerin 上调了 MAC-T 细胞中 CMKLR1、CCRL2 以及与脂肪酸合成、葡萄糖摄取、胰岛素信号传导和酪蛋白合成相关的基因的转录表达。催乳激素（胰岛素、生长激素和催乳素）下调 MAC-T 细胞中 CMKLR1 的表达。脂联素抑制 CMKLR1 表达。 TNF-α 抑制 CMKLRI，但诱导 CCRL2 表达。这些数据表明凯莫瑞是一种通过其自身受体在牛乳腺上皮细胞中调节泌乳的新型调节剂。 (C) 2015 Elsevier Inc. 保留所有权利。
Chemerin is a chemoattractant cytokine (chemokine) produced by adipocytes and hepatocytes; it regulates insulin sensitivity and adipocyte differentiation. The objective of this study was to investigate the effect of chemerin on the expression of genes related to lactogenesis and the regulators of chemerin signaling in a bovine mammary epithelial cell line (MAC-T). Two types of chemerin receptors, chemokine like-receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2), were detected in cultured MAC-T cells, whereas chemerin was not detected. G protein-coupled receptor 1 (GPR1), another receptor of chemerin, was undetectable in MAC-T cells. Chemerin upregulated transcript expression of CMKLR1, CCRL2, and genes associated with fatty acid synthesis, glucose uptake, insulin signaling, and casein synthesis in MAC-T cells. Lactogenic hormones (insulin, growth hormone, and prolactin) downregulated the expression of CMKLR1 in MAC-T cells. Adiponectin suppressed CMKLR1 expression. TNF-alpha suppressed CMKLRI, but induced CCRL2 expression. These data suggest chemerin is a novel regulator of lactogenesis via its own receptor in bovine mammary epithelial cells. (C) 2015 Elsevier Inc. All rights reserved.