Real time selective sequencing using nanopore technology.
Real time selective sequencing using nanopore technology.
复制标题
使用纳米孔技术的实时选择性测序。
DOI：
10.1101/038760
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Loose M
中科院分区：
文献类型：
--
作者：
Loose M
Oxford Nanopore Technologies MinION测序仪能够通过反转单个纳米孔的驱动电压来选择用于测序的特定DNA分子。为了直接选择用于测序的分子，我们使用动态时间规整来将读段与参考序列匹配。我们展示了我们的开源Read Until软件在小基因组内区域的实时选择性测序，单个扩增子富集和扩增子集的标准化。
The Oxford Nanopore Technologies MinION sequencer enables the selection of specific DNA molecules for sequencing by reversing the driving voltage across individual nanopores. To directly select molecules for sequencing, we used dynamic time warping to match reads to reference sequences. We demonstrate our open-source Read Until software in real-time selective sequencing of regions within small genomes, individual amplicon enrichment and normalization of an amplicon set.