Associations of atherosclerotic risk factors with oxidized low-density lipoprotein evaluated by LOX-1 ligand activity in healthy men
Associations of atherosclerotic risk factors with oxidized low-density lipoprotein evaluated by LOX-1 ligand activity in healthy men
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DOI：
10.1016/j.cca.2011.05.022
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发表时间：
2011-08-17
影响因子：
5
通讯作者：
Wakabayashi, Ichiro
中科院分区：
文献类型：
--
作者：
Uchida, Kagehiro;Suehiro, Akira;Wakabayashi, Ichiro
背景：本研究的目的是确定动脉粥样硬化危险因素与氧化低密度脂蛋白（OxLDL）的关系，通过一种新的酶免疫分析法来测量LOX-1（凝集素样OxLDL受体）配体。方法：研究对象为236名年龄33-62岁的健康男性。采用固定化重组LOX-1和抗apoB单克隆抗体对LOX-1配体含载脂蛋白ob （LAB）进行酶免疫测定。结果：简单回归分析，log- conversion LAB与吸烟史、腰围、舒张压、LDL胆固醇、log- conversion triglycerides、尿酸、白细胞计数呈显著正相关，与HDL胆固醇呈显著负相关。在以吸烟史、饮酒史、腰围、舒张压、高密度脂蛋白胆固醇、对数转换甘油三酯和尿酸为解释变量的多元回归分析中，对数转换LAB仅与吸烟史和对数转换甘油三酯存在显著相关性。重度吸烟者（>= 20支/天）的log -convert LAB显著高于非吸烟者和轻度吸烟者（= 150 mg/dl），大腰围（>= 85 cm），高舒张压（>= 85 mm Hg），或由NCEP-ATP III标准定义的代谢综合征的受试者，而不是没有这些危险因素或代谢综合征的受试者。结论：高甘油三酯血症和吸烟是健康男性LOX-1配体活性的决定因素，因此被认为是通过生成OxLDL引发动脉粥样硬化进展的关键危险因素。(C) 2011 Elsevier B.V.版权所有
Background: The aim of this study was to determine the relationships of risk factors for atherosclerosis with oxidized low-density lipoprotein (OxLDL) evaluated by a new enzyme immunoassay for measurement of LOX-1 (lectin-like OxLDL receptor) ligand.Methods: Subjects were 236 healthy men aged 33-62 years. LOX-1 ligand containing apoB (LAB) was measured by an enzyme-immunoassay using immobilized recombinant LOX-1 and anti-ApoB monoclonal antibody.Results: In simple regression analysis, log-converted LAB showed significant positive correlations with history of smoking, waist circumference, diastolic blood pressure, LDL cholesterol, log-converted triglycerides, uric acid and white blood cell count and showed a significant negative correlation with HDL cholesterol. In multiple regression analysis using history of smoking, history of drinking, waist circumference, diastolic blood pressure, HDL cholesterol, log-converted triglycerides and uric acid as explanatory variables, log-converted LAB showed significant correlations only with history of smoking and log-converted triglycerides. Log-converted LAB was significantly higher in heavy smokers (>= 20 cigarettes per day) than in nonsmokers and light smokers (= 150 mg/dl), large waist circumference (>= 85 cm), high diastolic blood pressure (>= 85 mm Hg), or metabolic syndrome defined by the NCEP-ATP III criteria than in subjects without each risk factor or metabolic syndrome.Conclusions: Hypertriglyceridemia and smoking are determinants of LOX-1 ligand activity in healthy men and are thus thought to be crucial risk factors for initiation of atherosclerotic progression through generation of OxLDL. (C) 2011 Elsevier B.V. All rights reserved.