Research Progress of Artificial Promoter in Plant Genetic Engineering
Research Progress of Artificial Promoter in Plant Genetic Engineering
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作者：
H. Zhen
启动子是一种DNA片段，通过允许RNA聚合酶和转录因子结合来调节基因转录。启动子也是基因工程中表达载体的重要组成部分。然而，天然启动子存在一些局限性，如表达活性低、特异性低。另外，人工构建的启动子有望克服这些限制，实现诱导因子更多、基础活性低、表达活性高、表达启动快等优势。本文从诱导表达、组织特异性表达和高效表达三个方面综述了人工启动子构建的研究进展。
Promoter is a piece of DNA fragment that regulates gene transcription by allowing RNA polymerase and transcription factors binding to it.Promoter is also an important element in the expression vectors in genetic engineering.However,there are some limitations in natural promoter,such as low expression activity and low specificity.Alternatively,artificial constructed promoters are expected to overcome those limits and achieve advantages,such as more induction factors,low basal activity,high expression activity and quick expression initiation.In this paper,we reviewed research advances in artificial promoter constructions in terms of the inducible expression,tissue-specific expression and high-efficiency expression.