Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable
Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable
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DOI：
10.1034/j.1399-0039.2000.550409.x
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发表时间：
2000-04-01
期刊：
影响因子：
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通讯作者：
Setterholm, M
中科院分区：
文献类型：
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作者：
Hurley, CK;Maiers, M;Setterholm, M
使用序列特异性寡核苷酸引物和/或探针对HLA-A和HLA-B基因座的HLA I类等位基因进行基于DNA的分型已被用于国家骨髓捐献者计划（R）无关捐献者登记处的大规模个体分型。分型由16个实验室在低分辨率水平下进行（例如A*01，B*07）。项目前12个月的盲法质量控制分析结果表明，分型高度准确、特异和可靠。基于11，545个HLA-A和11，428个HLA-B分配的总错误率为HLA-A的1.1%和HLA-B的1.9%。这一准确度尤其显著，因为无法将质量控制样本与实验室同时检测的64 180份供体样本区分开来。
DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program(R) unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A*01, B*07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rare based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories.