Multidimensional Clinical Surveillance of Pseudomonas aeruginosa Reveals Complex Relationships between Isolate Source, Morphology, and Antimicrobial Resistance.
Multidimensional Clinical Surveillance of Pseudomonas aeruginosa Reveals Complex Relationships between Isolate Source, Morphology, and Antimicrobial Resistance.
复制标题
铜绿假单胞菌的多维临床监测揭示了分离株来源、形态学和抗菌素耐药性之间的复杂关系。
DOI：
10.1128/msphere.00393-21
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发表时间：
2021-08-25
期刊：
影响因子：
4.8
通讯作者：
Papin JA
中科院分区：
文献类型：
--
作者：
Dunphy LJ;Kolling GL;Jenior ML;Carroll J;Attai AE;Farnoud F;Mathers AJ;Hughes MA;Papin JA
铜绿假单胞菌的抗菌药物敏感性取决于宿主和病原体特异性因素的复杂组合。通过对来自590名患者的971株临床铜绿假单胞菌分离株的分析和配对患者元数据的收集，我们表明抗菌素耐药性不仅与以患者为中心的因素（例如，囊性纤维化和抗假单胞菌处方史）以及微生物特异性表型（例如，粘液样菌落形态学）。此外，来自不同来源的分离株（例如，呼吸道、泌尿道）显示出与来源特异性抗菌药物处方策略相关的抗菌药物耐药性率。此外，来自同一患者的分离株往往表现出高度的异质性，突出了传染病个性化治疗面临的关键挑战。我们的研究结果支持隔离和患者水平数据集之间的新关系，为未来的抗菌治疗策略提供了潜在的指导。重要。铜绿假单胞菌是医院感染和囊性纤维化患者感染的主要原因。虽然铜绿假单胞菌感染和治疗可能因各种抗菌素耐药性和毒力机制而复杂化，但在临床环境中很少记录病原体毒力。在这项研究中，我们发现了抗生素耐药性，毒力相关的形态，并在一个大的和可变的临床铜绿假单胞菌分离株的集合分离源之间的新关系。我们的工作促进了对与毒力相关的铜绿假单胞菌形态的临床监测，以及对来源特异性抗菌药物处方和耐药模式的跟踪。
Antimicrobial susceptibility in Pseudomonas aeruginosa is dependent on a complex combination of host and pathogen-specific factors. Through the profiling of 971 clinical P. aeruginosa isolates from 590 patients and collection of paired patient metadata, we show that antimicrobial resistance is associated with not only patient-centric factors (e.g., cystic fibrosis and antipseudomonal prescription history) but also microbe-specific phenotypes (e.g., mucoid colony morphology). Additionally, isolates from different sources (e.g., respiratory tract, urinary tract) displayed rates of antimicrobial resistance that were correlated with source-specific antimicrobial prescription strategies. Furthermore, isolates from the same patient often displayed a high degree of heterogeneity, highlighting a key challenge facing personalized treatment of infectious diseases. Our findings support novel relationships between isolate and patient-level data sets, providing a potential guide for future antimicrobial treatment strategies. IMPORTANCEP. aeruginosa is a leading cause of nosocomial infection and infection in patients with cystic fibrosis. While P. aeruginosa infection and treatment can be complicated by a variety of antimicrobial resistance and virulence mechanisms, pathogen virulence is rarely recorded in a clinical setting. In this study, we discovered novel relationships between antimicrobial resistance, virulence-linked morphologies, and isolate source in a large and variable collection of clinical P. aeruginosa isolates. Our work motivates the clinical surveillance of virulence-linked P. aeruginosa morphologies as well as the tracking of source-specific antimicrobial prescription and resistance patterns.