Rates in vitro changes of gonococcal colony opacity phenotypes
Rates in vitro changes of gonococcal colony opacity phenotypes
复制标题
淋球菌菌落混浊表型的体外变化率
作者：
L. Mayer
测定了 12 种淋病奈瑟菌菌株的菌落不透明表型的变化率。平均变化率约为每代每个集落形成单位2 X 10(-3)，范围为每代每个集落形成单位0.2 X 10(-3)至4 X 10(-3)。从不透明到透明的转变与从透明到不透明的转变以相同的速率发生。以下因素对转化率没有影响：(i) 毛发状态； (ii) 作为特定表型的传代次数； (iii) 生长过程中温度、pH 值或大气中氧气含量的变化； (iv) 在生长培养基中添加 194 种化合物或混合物中的任何一种； (v) 添加 DNase 或来自不透明或透明淋球菌的 DNA； (vi)可见不透明-透明转变与导致毛发脱落的变化之间的潜伏。讨论了高转变率的一些影响。
The rate of change of colony opacity phenotype was determined for 12 strains of Neisseria gonorrhoeae. The average rate of change was about 2 X 10(-3) per colony-forming unit per generation with a range of 0.2 X 10(-3) to 4 X 10(-3) per colony-forming unit per generation. Transition from opaque to transparent occurred at the same rate as transition from transparent to opaque. The following factors were shown to have no effect on the transition rate: (i) the state of piliation; (ii) the number of passages as a particular phenotype; (iii) alteration in the temperature, pH, or amount of oxygen in the atmosphere during growth; (iv) the addition of any of 194 compounds or mixtures to the growth media; (v) the addition of DNase or of DNA from opaque or transparent gonococci; and (vi) incubation between the opacity-transparency transition and the change resulting in the loss of piliation was seen. Some implications of the high transition rate are discussed.