MID1, A NOVEL SACCHAROMYCES-CEREVISIAE GENE ENCODING A PLASMA-MEMBRANE PROTEIN, IS REQUIRED FOR CA2+ INFLUX AND MATING
MID1, A NOVEL SACCHAROMYCES-CEREVISIAE GENE ENCODING A PLASMA-MEMBRANE PROTEIN, IS REQUIRED FOR CA2+ INFLUX AND MATING
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DOI：
10.1128/mcb.14.12.8259
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发表时间：
1994-12-01
影响因子：
5.3
通讯作者：
ANRAKU, Y
中科院分区：
文献类型：
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作者：
IIDA, H;NAKAMURA, H;ANRAKU, Y
通过建立一种独特的筛选方法，我们已经分离出酵母突变体，这些突变体仅在分化成具有交配投射的细胞后死亡，并且其中一些突变体在Ca 2+信号传导中也有缺陷。突变体被分为五个互补组，其中之一，我们进行了广泛的研究。这种突变定义了一个新的基因，命名为p53，它编码一个N-糖基化的，完整的质膜蛋白与548个氨基酸残基。mid 1 -1突变体具有低的Ca 2+摄取活性，在接受交配信息素后失去活力，并且在与高浓度的CaCl 2孵育时逃脱死亡。该基因对营养生长不是必需的。MATa mid 1 -1和MAT alpha mid 1 -1细胞之间的交配效率较低。这些结果表明，Ca 2+内流和交配需要Ca 2+。
By establishing a unique screening method, we have isolated yeast mutants that die only after differentiating into cells with a mating projection, and some of them are also defective in Ca2+ signaling. The mutants were classified into five complementation groups, one of which we studied extensively. This mutation defines a new gene, designated MIDI, which encodes an N-glycosylated, integral plasma membrane protein with 548 amino acid residues. The mid1-1 mutant has low Ca2+ uptake activity, loses viability after receiving mating pheromones, and escapes death when incubated with high concentrations of CaCl2. The MIDI gene is nonessential for vegetative growth. The efficiency of mating between MATa mid1-1 and MAT alpha mid1-1 cells is low. These results demonstrate that MIDI is required for Ca2+ influx and mating.