Synergistic enhancement of H2O2 production in human epidermoid carcinoma cells by Benzo[a]pyrene and ultraviolet A radiation.
Synergistic enhancement of H2O2 production in human epidermoid carcinoma cells by Benzo[a]pyrene and ultraviolet A radiation.
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DOI：
10.1016/s0041-008x(03)00018-8
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发表时间：
2003-04
影响因子：
3.8
通讯作者：
E. Shyong;Yuhun Lu;A. Goldstein;M. Lebwohl;Huachen Wei
中科院分区：
文献类型：
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作者：
E. Shyong;Yuhun Lu;A. Goldstein;M. Lebwohl;Huachen Wei
苯并[a]芘（BaP）是一种普遍存在的环境污染物，具有潜在的致癌性。结果表明，BaP 在 UV A 照射下增强了纯化 DNA 和培养细胞中 8-羟基-2'-脱氧鸟苷的形成。本研究的目的是确定 BaP 和紫外线辐射是否协同产生活性氧 (ROS)，从而导致 DNA 碱基氧化。在这项研究中，测量了用 BaP 加紫外线辐射处理的 A431 细胞和原代人角质形成细胞中 H2O2 的水平，作为 ROS 的指标。用 BaP 加 UVB 或 UVA 处理的细胞产生的 H2O2 显着增加，其中后者的效果更大。 A431 细胞和原代人角质形成细胞对 BaP 和 UVA 照射的反应在细胞外 H2O2 的产生方面相似。此外，H2O2 的产生与 UVA 和 UVB 剂量成比例相关，但与时间或 BaP 浓度无关。用过氧化氢酶和一般ROS清除剂处理显着降低了用BaP加UVA处理的细胞的H2O2产生，而·O2−、·OH和1O2清除剂的影响最小。这些结果表明，BaP 通过 UVA 协同增强培养细胞中 H2O2 的产生，并在较小程度上通过 UVB，支持这样的假设：BaP 和 UVA 的相互作用可以产生 ROS，并进一步证实可能导致致癌的氧化性 DNA 损伤。
Benzo[a]pyrene (BaP) is an ubiquitous environmental pollutant with potential carcinogenecity. It was shown that BaP, upon irradiation by UV A, enhanced the formation of 8-hydroxy-2′-deoxyguanosine in purified DNA and in cultured cells. The purpose of this present study was to determine whether BaP and UV radiation synergistically generate reactive oxygen species (ROS) that consequently result in the oxidation of DNA bases. In this study, the levels of H2O2were measured as an indicator of ROS in A431 cells and primary human keratinocytes treated with BaP plus UV radiation. Production of H2O2significantly increased from cells treated with BaP plus UVB or UVA, with the latter having a much greater effect. The responses of A431 cells and primary human keratinocytes to BaP and UVA irradiation were similar in generation of extracellular H2O2. Also, H2O2production proportionally correlated with UVA and UVB dose, but was independent of time or BaP concentration. Treatment with catalase and general ROS scavengers significantly decreased H2O2production from cells treated with BaP plus UVA, whereas scavengers of•O2−,•OH, and1O2had minimal effects. These results demonstrate that BaP synergistically enhances the production of H2O2from cultured cells by UVA and, to a lesser extent, by UVB, supporting the hypothesis that interaction of BaP and UVA can generate ROS and further substantiate oxidative DNA damage that may lead to carcinogenesis.