A diffusible signal attracts olfactory sensory axons toward their target in the developing brain of the moth.
A diffusible signal attracts olfactory sensory axons toward their target in the developing brain of the moth.
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一种可扩散的信号将嗅觉感觉轴突吸引到飞蛾发育中的大脑中的目标。
DOI：
10.1002/neu.10210
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Buckingham,Jocelyn
中科院分区：
文献类型：
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作者：
Oland,LynneA;Pott,WendyM;Howard,CharlesT;Inlow,Mark;Buckingham,Jocelyn
嗅觉受体轴突用来导航到中枢神经系统中的目标的信号仍然没有很好的理解。在蛾Manduca sexta，初级嗅觉通路的发育胚后，和受体轴突导航从实验上可访问的感觉上皮细胞的大脑沿着一条通路足够长的详细研究的区域，轴突行为的变化。目前的实验询问扩散因子是否有助于受体轴突导向。从触角感受器上皮细胞制备外植体，并在胶原凝胶基质中与脑不同区域的切片共培养。受体轴突被吸引到大脑的中心区域，包括前脑和触角叶。受体轴突生长到一个切片的最接近的区域的触角神经，轴突分类通常发生，没有表现出方向偏好。当触角叶包括在切片中时，进入分选区的受体轴突直接朝向触角叶生长。与先前的体内实验相结合，目前的结果表明，一个有吸引力的扩散因子可以作为一个线索，将错误路由的嗅觉受体轴突导向大脑的内侧区域，在那里，局部线索将它们引导到触角叶。他们还表明，在正常情况下，受体轴突沿着预先存在的蛹神经到达触角叶，从叶发出的扩散因子平行且在短距离内起作用，以保持进入触角叶的路径的保真度。© 2003 Wiley Periodicals，Inc. J Neurobiol 56：24-40，2003
The signals that olfactory receptor axons use to navigate to their target in the CNS are still not well understood. In the mothManduca sexta, the primary olfactory pathway develops postembryonically, and the receptor axons navigate from an experimentally accessible sensory epithelium to the brain along a pathway long enough for detailed study of regions in which axon behavior changes. The current experiments ask whether diffusible factors contribute to receptor axon guidance. Explants were made from the antennal receptor epithelium and co‐cultured in a collagen gel matrix with slices of various regions of the brain. Receptor axons were attracted toward the central regions of the brain, including the protocerebrum and antennal lobe. Receptor axons growing into a slice of the most proximal region of the antennal nerve, where axon sorting normally occurs, showed no directional preference. When the antennal lobe was included in the slice, the receptor axons entering the sorting region grew directly toward the antennal lobe. Taken together with the previousin vivoexperiments, the current results suggest that an attractive diffusible factor can serve as one cue to direct misrouted olfactory receptor axons toward the medial regions of the brain, where local cues guide them to the antennal lobe. They also suggest that under normal circumstances, in which the receptor axons follow a pre‐existing pupal nerve to the antennal lobe, the diffusible factor emanating from the lobe acts in parallel and at short range to maintain the fidelity of the path into the antennal lobe. © 2003 Wiley Periodicals, Inc. J Neurobiol 56: 24–40, 2003