CLONING AND SEQUENCING OF THE CDNA FOR HUMAN MONOCYTE CHEMOTACTIC AND ACTIVATING FACTOR (MCAF)
CLONING AND SEQUENCING OF THE CDNA FOR HUMAN MONOCYTE CHEMOTACTIC AND ACTIVATING FACTOR (MCAF)
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DOI：
10.1016/0006-291x(89)92430-3
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发表时间：
1989-02-28
影响因子：
3.1
通讯作者：
MATSUSHIMA, K
中科院分区：
文献类型：
--
作者：
FURUTANI, Y;NOMURA, H;MATSUSHIMA, K
分离和测序了具有编码人单核细胞趋化和激活因子(MCAF)的核苷酸序列的cDNA克隆。由核苷酸序列推导出的氨基酸序列揭示了MCAF前体的一级结构，它由23个氨基酸残基组成的信号肽序列和76个氨基酸残基组成的成熟MCAF序列组成。MCAF的氨基酸序列与诱导细胞因子家族的其他成员有25%-55%的同源性，包括巨噬细胞炎症蛋白和一些可能的多肽介质JE、LD78、RANTES和TCA-3。这表明MCAF是参与免疫和炎症反应的因子家族的一员。
cDNA clones having a nucleotide sequence encoding a human monocyte chemotactic and activating factor (MCAF) were isolated and sequenced. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. The amino acid sequence of MCAF showed 25-55% homology with other members of an inducible cytokine family, including macrophage inflammatory protein and some putative polypeptide mediators known as JE, LD78, RANTES and TCA-3. This suggests that MCAF is a member of family of factors involved in immune and inflammatory responses.