The PKR regulatory subunit of protein kinase A (PKA) is involved in the regulation of growth, sexual and asexual development, and pathogenesis in Fusarium graminearum
The PKR regulatory subunit of protein kinase A (PKA) is involved in the regulation of growth, sexual and asexual development, and pathogenesis in Fusarium graminearum
复制标题
蛋白激酶 A (PKA) 的 PKR 调节亚基参与禾谷镰刀菌生长、有性和无性发育以及发病机制的调节
DOI：
10.1111/mpp.12576
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发表时间：
2018-04-01
影响因子：
4.9
通讯作者：
Wang, Chenfang
中科院分区：
文献类型：
--
作者：
Li, Chaoqun;Zhang, Yonghui;Wang, Chenfang
禾谷镰刀菌（Fusarium graminearum）是小麦赤霉病的病原菌，也是脱氧雪腐镰刀菌烯醇（DON）毒素的产生者。用外源性环磷酸腺苷（cAMP）处理增加其DON产生。为了更好地了解cAMP-蛋白激酶A（PKA）通路在F。禾谷镰刀菌中，我们的功能特点PKR基因编码的PKA调节亚基。缺失PKR的突变体是可行的，但表现出严重的缺陷，生长，产孢和植物感染。pkr突变体产生紧凑的菌落，气生菌丝较短，菌丝室中的细胞核数量增加。突变分生孢子形态异常，似乎经历快速自噬相关的细胞死亡。pkr突变体表现出囊壳发育受阻，但DON产量增加。它的病情指数小于1，未能传播到邻近的小穗。该突变体是不稳定的和自发的抑制剂具有更快的增长速度往往产生旧的文化。共分离出67株生长速度比原始突变体快的抑制菌株。其中三个表现出与野生型相似的生长速率和菌落形态，但仍然存在分生孢子缺陷。在三个代表性的抑制菌株的18个候选PKA相关基因的测序分析确定的突变，只有在CPK 1催化亚基基因。进一步的鉴定表明，其他64个抑制菌株中有10个也有CPK 1突变。这些结果表明，PKR在菌丝生长、繁殖、致病和DON产生等过程中起着重要的调控作用，而CPK 1的突变部分抑制了PKR的缺失。禾谷早熟禾
Fusarium graminearum is a causal agent of wheat scab disease and a producer of deoxynivalenol (DON) mycotoxins. Treatment with exogenous cyclic adenosine monophosphate (cAMP) increases its DON production. In this study, to better understand the role of the cAMP-protein kinase A (PKA) pathway in F. graminearum, we functionally characterized the PKR gene encoding the regulatory subunit of PKA. Mutants deleted of PKR were viable, but showed severe defects in growth, conidiation and plant infection. The pkr mutant produced compact colonies with shorter aerial hyphae with an increased number of nuclei in hyphal compartments. Mutant conidia were morphologically abnormal and appeared to undergo rapid autophagy-related cell death. The pkr mutant showed blocked perithecium development, but increased DON production. It had a disease index of less than unity and failed to spread to neighbouring spikelets. The mutant was unstable and spontaneous suppressors with a faster growth rate were often produced on older cultures. A total of 67 suppressor strains that grew faster than the original mutant were isolated. Three showed a similar growth rate and colony morphology to the wild-type, but were still defective in conidiation. Sequencing analysis with 18 candidate PKA-related genes in three representative suppressor strains identified mutations only in the CPK1 catalytic subunit gene. Further characterization showed that 10 of the other 64 suppressor strains also had mutations in CPK1. Overall, these results showed that PKR is important for the regulation of hyphal growth, reproduction, pathogenesis and DON production, and mutations in CPK1 are partially suppressive to the deletion of PKR in F. graminearum.