Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: A microscopic overview
Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: A microscopic overview
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DOI：
10.1021/la0513712
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发表时间：
2005-11-08
期刊：
影响因子：
3.9
通讯作者：
Sastry, M
中科院分区：
文献类型：
--
作者：
Shukla, R;Bansal, V;Sastry, M
巨噬细胞是主要的免疫效应细胞之一，在免疫系统中作为分泌细胞、吞噬细胞和抗原呈递细胞发挥重要作用。在这项研究中，我们解决了这个问题的细胞毒性和免疫原性影响的金纳米粒子对RAW 264.7巨噬细胞。金纳米颗粒的细胞毒性已经与使用各种显微镜工具如原子力显微镜（AFM）、共聚焦激光扫描显微镜（CFLSM）和透射电子显微镜（TEM）对其内吞摄取的详细研究相关。我们的研究结果表明，Au（0）纳米颗粒没有细胞毒性，减少活性氧和亚硝酸盐的产生，并不引起促炎细胞因子TNF-α和IL-1的分泌，使它们成为纳米医学的合适候选者。AFM测量表明，金纳米粒子通过涉及胞饮作用的机制内化在细胞内，而CFLSM和TEM研究表明它们内化在以核周方式排列的溶酶体体中。因此，我们的研究强调了非细胞毒性，非免疫原性和生物相容性的性质ofgold纳米粒子与应用在nanoimmunology，nanomedicine和nanobiotechnology的潜力。
Macrophages are one of the principal immune effector cells that play essential roles as secretory, phagocytic, and antigen-presenting cells in the immune system. In this study, we address the issue of cytotoxicity and immunogenic effects of gold nanoparticles on RAW264.7 macrophage cells. The cytotoxicity of gold nanoparticles has been correlated with a detailed study of their endocytotic uptake using various microscopy tools such as atomic force microscopy (AFM), confocal-laser-scanning microscopy (CFLSM), and transmission electron microscopy (TEM). Our findings suggest that Au(0) nanoparticles are not cytotoxic, reduce the production of reactive oxygen and nitrite species, and do not elicit secretion of proinflammatory cytokines TNF-alpha and IL1-, making them suitable candidates for nanomedicine. AFM measurements suggest that gold nanoparticles are internalized inside the cell via a mechanism involving pinocytosis, while CFLSM and TEM studies indicate their internalization in lysosomal bodies arranged in perinuclear fashion. Our studies thus underline the noncytotoxic, nonimmunogenic, and biocompatible properties ofgold nanoparticles with the potential for application in nanoimmunology, nanomedicine, and nanobiotechnology.