Synchronous release of ATP and neurotransmitter within milliseconds of a motor nerve impulse in the frog.
Synchronous release of ATP and neurotransmitter within milliseconds of a motor nerve impulse in the frog.
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青蛙运动神经冲动发生后几毫秒内同步释放 ATP 和神经递质。
DOI：
10.1113/jphysiol.1996.sp021348
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Redman,RS
中科院分区：
文献类型：
--
作者：
Silinsky,EM;Redman,RS
1.有人认为，ATP与神经递质乙酰胆碱（ACh）一起释放，水解为腺苷后，是连接前神经肌肉抑制的主要生理介质。为了评价ATP是否以足够快的速度释放以介导连接前抑制，从急性分离的豚鼠交感神经元制备含有ATP门控和ACh门控离子通道的外向贴片，并用于检测青蛙皮心神经肌肉制剂中核苷酸和神经递质的共同释放。2.在一个正常的沐浴溶液中，肌肉烟碱受体被阻断，一个单一的刺激运动神经产生通道开放的检测器补丁的特点ATP和乙酰胆碱。3.在其余的实验中，用足够的六甲铵（200 μ M）处理制剂，以阻断检测器贴片中的烟碱反应。在这些实验中，当贴片放置在运动神经末梢的10微米内时，单个暂时隔离的神经冲动导致检测器贴片中ATP门控通道的同步打开，延迟< 5 ms。这种多通道阶段性反应之后是ATP门控通道特征性的离散通道开放的踪迹。4.选择性ATP拮抗剂苏拉明（50 μ M）可逆地消除了对神经刺激的反应。5.结果表明，ATP的同步释放与神经递质ACh在响应于一个单独的神经冲动和短暂的潜伏期特征的量子释放从突触囊泡。
1. It has been suggested that ATP is released together with the neurotransmitter acetylcholine (ACh) and, after hydrolysis to adenosine, is the primary physiological mediator of prejunctional neuromuscular depression. To evaluate whether ATP is released with sufficient rapidity to mediate prejunctional depression, outside‐out patches containing both ATP‐gated and ACh‐gated ion channels were made from acutely dissociated guinea‐pig sympathetic neurons and used to detect the co‐release of nucleotide and neurotransmitter in frog cutaneous pectoris nerve‐muscle preparations. 2. In a normal bathing solution in which muscle nicotinic receptors were blocked, a single stimulus to the motor nerve produced channel openings in the detector patch characteristic of both ATP and ACh. 3. In the remaining experiments, preparations were treated with sufficient hexamethonium (200 microM) to block nicotinic responses in the detector patch. In these experiments, a single temporally isolated nerve impulse caused the synchronous opening of ATP‐gated channels in the detector patch with a latency of < 5 ms when patches were placed within 10 microns of the motor nerve ending. This multichannel phasic response was followed by trail of discrete channel openings characteristic of ATP‐gated channels. 4. The selective ATP antagonist suramin (50 microM) reversibly eliminated the response to nerve stimulation. 5. The results suggest that ATP is released synchronously together with the neurotransmitter ACh in response to an individual nerve impulse and with a brief latency characteristic of quantal release from synaptic vesicles.
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DOI：
--
发表时间：
2005
期刊：
--
影响因子：
--
作者：
Bertil;-B.;Fredholm;Maria;-P.;Abbracchio;Geoffrey;Burnstock;John;-W.;Daly;-T.;Kendall
通讯作者：
Bertil;-B.;Fredholm;Maria;-P.;Abbracchio;Geoffrey;Burnstock;John;-W.;Daly;-T.;Kendall
选择性腺苷拮抗剂（8-环戊基-1,3-二丙基黄嘌呤）通过作用于 A1 受体消除神经肌肉抑制和外源腺苷的作用。
影响因子：
3.6
作者：
Redman,RS;Silinsky,EM
通讯作者：
Silinsky,EM
ATP 与乙酰胆碱一起释放，作为青蛙运动神经末梢神经肌肉抑制的介质。
DOI：
10.1113/jphysiol.1994.sp020176
发表时间：
1994
期刊：
The Journal of physiology
影响因子：
--
作者：
Redman,RS;Silinsky,EM
通讯作者：
Silinsky,EM
胆碱能神经末梢储存和释放乙酰胆碱：非哺乳动物制剂的最新结果。
DOI：
--
发表时间：
1972
期刊：
Biochemical Society Symposium
影响因子：
--
作者：
Whittaker Vp;M. Dowdall;A. Boyne
通讯作者：
A. Boyne
关于递质分泌与哺乳动物运动神经末梢释放腺嘌呤核苷酸之间的关联。
DOI：
--
发表时间：
1975
期刊：
Journal of Physiology
影响因子：
--
作者：
E. M. Silinsky
通讯作者：
E. M. Silinsky