Overexpression of TEAD4 correlates with poor prognosis of glioma and promotes cell invasion
Overexpression of TEAD4 correlates with poor prognosis of glioma and promotes cell invasion
复制标题
TEAD4的过度表达与神经胶质瘤的不良预后相关并促进细胞侵袭
DOI：
--
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ye Song
中科院分区：
文献类型：
--
作者：
Anqi Xu;Xizhao Wang;Yu Zeng;Mingfeng Zhou;Renhui Yi;Zhiyong Wu;Jie Lin;Ye Song
本研究旨在揭示TEA结构域转录因子4(TEAD4)在脑胶质瘤中表达升高与疾病预后的关系。收集GEO数据库(GSE4290)中TEAD4基因在胶质瘤中的表达数据，用免疫印迹和免疫组织化学方法证实TEAD4蛋白在胶质瘤中的表达。采用Kaplan-Meier分析和对数等级检验，揭示TEAD4表达水平与患者生存的相关性。用Transwell法和Boyden法分别检测TEAD4对细胞迁移和侵袭的影响。基因集浓缩分析(GSEA)预测TEAD4在脑胶质瘤中可能的生物学功能。结果表明，与正常脑组织相比，脑胶质瘤组织中TEAD4的mRNA和蛋白表达上调。此外，TEAD4的过度表达与脑胶质瘤患者的预后不良有关。TEAD4基因敲除可显著抑制胶质瘤细胞的体外迁移和侵袭。TEAD4基因敲除导致N-钙粘蛋白、波形蛋白和Slug表达下调，而E-钙粘蛋白表达上调，这与Gesa发现TEAD4与上皮-间充质转化密切相关。我们的研究表明，TEAD4的过度表达可能是脑胶质瘤中一个潜在的不良预后指标。TEAD4基因敲除导致胶质瘤迁移和侵袭受到抑制
This study aimed to reveal the correlation of increased TEA domain transcription factor 4 (TEAD4) expression and disease prognosis in glioma. The expression data of TEAD4 mRNA in glioma were collected from GEO database (GSE4290), and the expression of TEAD4 protein in glioma was confirmed using western blot and Immunohistochemistry. Kaplan-Meier analysis with the log-rank test was used to reveal the correlation of TEAD4 expression level and patients’ survival. The effects of TEAD4 on migration and invasion were separately examined by Transwell assay and Boyden assay. Gene set enrichment analysis (GSEA) was performed to predict the possible biological function of TEAD4 in glioma. The results showed that TEAD4 mRNA and protein expression were upregulated in glioma tissues compared to normal brain tissues. Furthermore, overexpression of TEAD4 correlated with poor prognosis in glioma patients. Knockdown of TEAD4 markedly inhibited glioma cells migration and invasion in vitro. Consistent with the result that TEAD4 was associated with epithelial-mesenchymal transition (EMT) closely by GESA, knockdown of TEAD4 resulted in N-cadherin, vimentin and Slug downregulated but E-cadherin upregulated. Our study indicated that overexpression of TEAD4 may represent as a potential unfavorable marker for poor survival and prognosis in glioma. Knockdown of TEAD4 led to suppressed glioma migration and invasion