cDNA micro array identification of a gene differentially expressed in adenovirus type 5‐ versus type 12‐transformed cells
cDNA micro array identification of a gene differentially expressed in adenovirus type 5‐ versus type 12‐transformed cells
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cDNA 微阵列鉴定 5 型腺病毒与 12 型腺病毒转化细胞中差异表达的基因
DOI：
10.1016/s0014-5793(00)02339-5
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发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
A. Zantema
中科院分区：
文献类型：
--
作者：
A. Vertegaal;H. Kuiperij;T. van Laar;V. Scharnhorst;A. van der Eb;A. Zantema
由5型非致癌腺病毒和12型致癌腺病毒编码的蛋白质差异地影响许多细胞基因的表达。我们使用cDNA微阵列分析来鉴定在Ad 12中表达但在Ad 5转化细胞中不表达的细胞基因。该细胞基因被发现是编码卵泡抑素相关蛋白的基因，一种TGF-β诱导基因。因此，在腺病毒12型转化细胞中发现了与Smad结合元件结合的组成性因子。
Proteins encoded by non‐oncogenic adenovirus type 5 and oncogenic adenovirus type 12 differentially affect expression of a number of cellular genes. We have used cDNA micro array analysis to identify a cellular gene that is expressed in Ad12‐ but not in Ad5‐transformed cells. This cellular gene was found to be the gene encoding follistatin‐related protein, a TGF‐β inducible gene. Consistently, a constitutive factor binding to Smad binding elements was found in adenovirus type 12‐transformed cells.