Integrated Biocatalytic Platform Based on Aqueous Biphasic Systems for the Sustainable Oligomerization of Rutin
Integrated Biocatalytic Platform Based on Aqueous Biphasic Systems for the Sustainable Oligomerization of Rutin
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DOI：
10.1021/acssuschemeng.1c03399
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发表时间：
2021-07-14
影响因子：
8.4
通讯作者：
Eibes, Gemma
中科院分区：
文献类型：
--
作者：
Muniz-Mouro, Abel;Ferreira, Ana M.;Eibes, Gemma
鲁丁蛋白是一种已知的抗氧化剂化合物，它显示出广泛的生物学活性和与健康相关的益处，但具有低水溶性，可以通过其聚合来克服。在这项工作中，研究了由离子液体胆碱磷酸二氢（[CH] [DHPH]）和聚合物聚（PEG 600）600（PEG 600）组成的生物相容性水。 - 鲁丁蛋白的寡聚化。研究了两种不同的方法，以在几个寡聚蛋白生产周期中重复使用漆酶，它们之间的主要区别在于，它们在低聚反应过程中使用单相或双相状态。在第二种方法（异质反应培养基）中使用双相状态允许在三个连续的反应分离循环中成功再利用生物催化剂，同时达到值得注意的鲁丁素寡聚率（在第一个周期中为91％，在第二个周期中为91％，在第二个周期中为91％，为95％在最后一个周期中为89％）。这些显着的结果是由鲁丁蛋白在PEG富相中的溶解度增加以及[CH] [DHPH] - 富含量的漆酶的增强催化性能的结合，以及对反应的pH值的优化而引起的。中等与酶稳定性直接链接。最后，进行了生命周期评估，以将这个综合反应分离平台与三个替代过程进行比较，从而增强了其可持续性。
Rutin is a known antioxidant compound that displays a broad range of biological activities and health-related benefits but presents a low water solubility that can be overcome by its polymerization. In this work, biocompatible aqueous biphasic systems composed of the ionic liquid cholinium dihydrogen phosphate ([CH][DHph]) and the polymer poly(ethylene glycol) 600 (PEG 600) were investigated as an efficient integrated reaction-separation platform for the laccase-catalyzed oligomerization of rutin. Two different approaches were studied to reuse laccase in several oligorutin production cycles, the main difference between them being the use of monophasic or biphasic regimes during the oligomerization reaction. The use of a biphasic regime in the second approach (heterogeneous reaction medium) allowed the successful reuse of the biocatalyst in three consecutive reaction-separation cycles while achieving noteworthy rutin oligomerization yields (95% in the first cycle, 91% in the second cycle, and 89% in the last cycle). These remarkable results were caused by the combination of the increased solubility of rutin in the PEG-rich phase together with the enhanced catalytic performance of laccase in the [Ch][DHph]-rich phase, alongside with the optimization of the pH of the reaction medium straightly linked to enzyme stability. Finally, a life-cycle assessment was performed to compare this integrated reaction-separation platform to three alternative processes, reinforcing its sustainability.