HOW TO ISOLATE CARDIAC MYOCYTES
HOW TO ISOLATE CARDIAC MYOCYTES
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DOI：
10.1093/cvr/28.2.280
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发表时间：
1994-02-01
影响因子：
10.8
通讯作者：
TYTGAT, J
中科院分区：
文献类型：
--
作者：
TYTGAT, J
一个完整的技术描述了从哺乳动物心脏使用Langendorff灌注技术的心肌细胞的分离。使用含有胶原酶和蛋白酶的无钙溶液，然后使用低钙溶液，一致地产生大量的钙耐受性肌细胞，其非常适合于长时间的电生理记录。
A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of electrophysiological recording.