Molecular interplay between microRNA-130a and PTEN in palmitic acid-mediated impaired function of endothelial progenitor cells: Effects of metformin
Molecular interplay between microRNA-130a and PTEN in palmitic acid-mediated impaired function of endothelial progenitor cells: Effects of metformin
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DOI：
10.3892/ijmm.2019.4140
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发表时间：
2019-05-01
影响因子：
5.4
通讯作者：
Shen, Fei-Xia
中科院分区：
文献类型：
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作者：
Gu, Xuemei;Wang, Xiao-Qian;Shen, Fei-Xia
甲硫氨酸在改善内皮祖细胞（EPCs）功能方面起重要作用。MicroRNA（miRNAs）是一类非编码小分子RNA，是内皮祖细胞血管功能的重要调控因子。本研究探讨二甲双胍和miRNA-130 a（miR-130 a）在棕榈酸（PA）作用下EPCs功能中的分子串扰。分别用二甲双胍、PA和二甲双胍+ PA处理分离的EPC。采用Cell Counting Kit-8、Transwell和Matrigel法检测不同处理后EPCs的增殖、迁移和管形成能力。采用逆转录-定量聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blotting）检测miR-130 a、磷酸酶和张力蛋白同源物（PTEN）及磷酸化AKT的表达。通过抑制miR-130 a模拟物和抑制剂分别在EPCs中过表达和抑制miR-130 a的表达，进一步阐明二甲双胍在EPCs中发挥作用的具体机制。EPCs增殖功能受损（P
Metformin serves an important role in improving the functions of endothelial progenitor cells (EPCs). MicroRNAs (miRNAs), small non-coding RNAs, have been investigated as significant regulators of EPC vascular functions. The present study investigated the molecular crosstalk between metformin and miRNA-130a (miR-130a) in the functions of EPCs exposed to palmitic acid (PA). Isolated EPCs were treated with metformin, PA, and metformin + PA, respectively. Cell Counting Kit-8, Transwell and Matrigel assays were performed to detect the proliferation, migration and tube formation ability of EPCs following different treatments. The expression of miR-130a, phosphatase and tensin homolog (PTEN) and phosphorylated-AKT was analyzed by reverse transcription-quantitative polymerase chain reaction and western blotting. The specific mechanism underlying the function of metformin in EPCs was further elucidated by transfecting miR-130a mimics and inhibitor to overexpress and inhibit the expression of miR-130a in EPCs, respectively. EPCs exhibited impaired functions of proliferation (P