Expression of Arabidopsis CAX2 in tobacco.: Altered metal accumulation and increased manganese tolerance
Expression of Arabidopsis CAX2 in tobacco.: Altered metal accumulation and increased manganese tolerance
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DOI：
10.1104/pp.124.1.125
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发表时间：
2000-09-01
期刊：
影响因子：
7.4
通讯作者：
Wagner, GJ
中科院分区：
文献类型：
--
作者：
Hirschi, KD;Korenkov, VD;Wagner, GJ
金属从细胞质溶胶到液泡的运输被认为是离子耐受性和植物修复潜力的重要组成部分。拟南芥反转运蛋白CAX2（钙交换器2）可能是这一过程的关键中介。酵母中CAX2的表达抑制了Ca2+和Mn2+的生长缺陷。一种针对反转运蛋白的肽特异性抗体与来自植物液泡膜的39-kD蛋白反应。表达CAX2的烟草植株积累了更多的Ca2+、Cd2+和Mn2+，对Mn2+水平升高的耐受性更强。CAX2在烟草中的表达增加了离体根质体囊泡中Cd2+和Mn2+的转运。这些结果表明，CAX2具有广泛的底物范围，对该转运体的调节可能是未来提高植物离子耐受性策略的重要组成部分。
Metal transport from the cytosol to the vacuole is thought to be an important component of ion tolerance and of a plant's potential for use in phytoremediation. The Arabidopsis antiporter CAX2 (calcium exchanger 2) may be a key mediator of this process. CAX2 expression in yeast suppressed both Ca2+ and Mn2+ growth defects. A peptide-specific antibody to the antiporter reacted with a 39-kD protein from plant vacuolar membranes. Tobacco (Nicotiana tabacum) plants expressing CAX2 accumulated more Ca2+, Cd2+,and Mn2+ and were more tolerant to elevated Mn2+ levels. Expression of CAX2 in tobacco increased Cd2+ and Mn2+ transport in isolated root tonoplast vesicles. These results suggest that CAX2 has a broad substrate range and modulation of this transporter may be an important component of future strategies to improve plant ion tolerance.