THERMOTROPIC PHASE-TRANSITIONS IN THE PLASMA-MEMBRANE OF RAM SPERMATOZOA
THERMOTROPIC PHASE-TRANSITIONS IN THE PLASMA-MEMBRANE OF RAM SPERMATOZOA
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DOI：
10.1530/jrf.0.0780447
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发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
NORTH, RD
中科院分区：
文献类型：
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作者：
HOLT, WV;NORTH, RD
使用膜探针1，6-二苯基-1，3，5-己三烯（DPH）的稳态荧光偏振技术表明，在17、26和36 °的区域中分别发生可检测的跃迁。绵羊精子质膜分离制备物中的C。基于钙和镁激活的ATP酶的温度相关行为的独立技术检测到在24 ℃的区域中的单一相变。C.从磷脂酶A2引起的酶的显着刺激的结果推断，相邻的脂质组合物的ATP酶的调制。富含胆固醇的脂质体引起相变温度从24 ℃上移。C至30度。C，但其原因尚不清楚。人们认为，这些相变可能对精子的存活和生理有深远的影响，无论是在正常的受精过程中，并在响应冷冻。
A steady-state fluorescence polarization technique, using the membrane probe 1,6-dipheny;-1,3,5-hexatriene (DPH), showed that separately detectable transitions occurred in the regions of 17, 26 and 36.degree. C in isolated preparations of ram sperm plasma membrane. An independent technique based on the temperature-related behavior of calcium- and magnesium-activated ATPase detected a single phase transition in the region of 24.degree. C. Modulation of ATPase by neighboring lipid composition was inferred from findings that phospholipase A2 caused significant stimulation of the enzyme. Cholesterol-rich liposomes caused an upward shift of the phase-transition temperature from 24.degree. C to 30.degree. C, but the reasons for this are unclear. It is considered that these phase transitions may have profound effects on sperm survival and physiology, both during normal fertilization processes and in response to cryostorage.